Detailed information NTG0781358

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PP479_RS02715
• Genome accession: NZ_CP116935
• Coordinates: 585721..586869 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain CUVET18-860
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 580721..591869

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PP479_RS02690 (PP479_02690)	-	581872..582168 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PP479_RS02695 (PP479_02695)	-	582165..582758 (-)	594	WP_003084544.1	YggT family protein	-
PP479_RS02700 (PP479_02700)	proC	582769..583590 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
PP479_RS02705 (PP479_02705)	-	583602..584294 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PP479_RS02710 (PP479_02710)	pilT	584509..585543 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PP479_RS02715 (PP479_02715)	pilU	585721..586869 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PP479_RS02720 (PP479_02720)	czcD	586877..587776 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PP479_RS02725 (PP479_02725)	-	587931..588335 (+)	405	WP_003084560.1	NINE protein	-
PP479_RS02730 (PP479_02730)	-	588578..589951 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
PP479_RS02735 (PP479_02735)	-	589948..591132 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=781358 PP479_RS02715 WP_003100959.1 585721..586869(+) (pilU) [Pseudomonas aeruginosa strain CUVET18-860]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=781358 PP479_RS02715 WP_003100959.1 585721..586869(+) (pilU) [Pseudomonas aeruginosa strain CUVET18-860]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382