Detailed information NTG0781326

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PP477_RS02180
• Genome accession: NZ_CP116934
• Coordinates: 473216..474364 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain CUVET20-956
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 468216..479364

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PP477_RS02155 (PP477_02155)	-	469368..469664 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PP477_RS02160 (PP477_02160)	-	469661..470254 (-)	594	WP_003084544.1	YggT family protein	-
PP477_RS02165 (PP477_02165)	proC	470265..471086 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PP477_RS02170 (PP477_02170)	-	471098..471789 (-)	692	Protein_432	YggS family pyridoxal phosphate-dependent enzyme	-
PP477_RS02175 (PP477_02175)	pilT	472004..473038 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PP477_RS02180 (PP477_02180)	pilU	473216..474364 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PP477_RS02185 (PP477_02185)	czcD	474372..475271 (-)	900	WP_003084557.1	CDF family zinc transporter CzcD	-
PP477_RS02190 (PP477_02190)	-	475426..475830 (+)	405	WP_003084560.1	NINE protein	-
PP477_RS02195 (PP477_02195)	-	476073..477446 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
PP477_RS02200 (PP477_02200)	-	477443..478627 (+)	1185	WP_003084564.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=781326 PP477_RS02180 WP_003100959.1 473216..474364(+) (pilU) [Pseudomonas aeruginosa strain CUVET20-956]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=781326 PP477_RS02180 WP_003100959.1 473216..474364(+) (pilU) [Pseudomonas aeruginosa strain CUVET20-956]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382