Detailed information NTG0781293

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PP478_RS02175
• Genome accession: NZ_CP116933
• Coordinates: 473071..474219 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain CUVET21-397
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 468071..479219

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PP478_RS02150 (PP478_02150)	-	469222..469518 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PP478_RS02155 (PP478_02155)	-	469515..470108 (-)	594	WP_003084544.1	YggT family protein	-
PP478_RS02160 (PP478_02160)	proC	470119..470940 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PP478_RS02165 (PP478_02165)	-	470952..471644 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PP478_RS02170 (PP478_02170)	pilT	471859..472893 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PP478_RS02175 (PP478_02175)	pilU	473071..474219 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PP478_RS02180 (PP478_02180)	czcD	474227..475126 (-)	900	WP_003084557.1	CDF family zinc transporter CzcD	-
PP478_RS02185 (PP478_02185)	-	475281..475685 (+)	405	WP_003084560.1	NINE protein	-
PP478_RS02190 (PP478_02190)	-	475928..477301 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
PP478_RS02195 (PP478_02195)	-	477298..478482 (+)	1185	WP_003084564.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=781293 PP478_RS02175 WP_003100959.1 473071..474219(+) (pilU) [Pseudomonas aeruginosa strain CUVET21-397]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=781293 PP478_RS02175 WP_003100959.1 473071..474219(+) (pilU) [Pseudomonas aeruginosa strain CUVET21-397]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382