Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   PNF30_RS17335 Genome accession   NZ_CP116774
Coordinates   3409765..3410193 (+) Length   142 a.a.
NCBI ID   WP_144472865.1    Uniprot ID   -
Organism   Bacillus safensis strain SRCM125915     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3404765..3415193
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PNF30_RS17325 (PNF30_17325) - 3405349..3407337 (-) 1989 WP_272510486.1 hydantoinase B/oxoprolinase family protein -
  PNF30_RS17330 (PNF30_17330) - 3407327..3409396 (-) 2070 WP_249856825.1 hydantoinase/oxoprolinase family protein -
  PNF30_RS17335 (PNF30_17335) nucA/comI 3409765..3410193 (+) 429 WP_144472865.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  PNF30_RS17340 (PNF30_17340) - 3410321..3412018 (+) 1698 WP_272510487.1 methyl-accepting chemotaxis protein -
  PNF30_RS17345 (PNF30_17345) - 3412139..3412525 (+) 387 WP_272510488.1 competence protein ComJ -
  PNF30_RS17350 (PNF30_17350) - 3412527..3413333 (-) 807 WP_272510489.1 aminoglycoside N(3)-acetyltransferase -
  PNF30_RS17355 (PNF30_17355) - 3413362..3413778 (-) 417 WP_048238129.1 Lrp/AsnC family transcriptional regulator -
  PNF30_RS17360 (PNF30_17360) - 3413894..3414658 (+) 765 WP_272510490.1 MBL fold metallo-hydrolase -
  PNF30_RS17365 (PNF30_17365) - 3414703..3415032 (-) 330 WP_197198033.1 YckD family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 15856.81 Da        Isoelectric Point: 5.9135

>NTDB_id=780205 PNF30_RS17335 WP_144472865.1 3409765..3410193(+) (nucA/comI) [Bacillus safensis strain SRCM125915]
MKLLKIILLLLLIVIGVATGYIKLEQGKQETANSSYDKTIHFPSDRYPETAKHIEEAIDEGHSSICTIDRKHSDEQRDQS
LHGVPTKRGYDRDEWPMAMCKEGGTGASVKYVSPSDNRGAGSWVSHQLSDDPDGTRIQFIID

Nucleotide


Download         Length: 429 bp        

>NTDB_id=780205 PNF30_RS17335 WP_144472865.1 3409765..3410193(+) (nucA/comI) [Bacillus safensis strain SRCM125915]
ATGAAACTTCTTAAAATCATTCTCTTGCTTTTATTAATTGTCATTGGTGTAGCAACAGGCTATATCAAGCTGGAGCAAGG
GAAACAAGAGACAGCGAATTCATCTTATGACAAAACCATTCACTTTCCATCAGATCGCTATCCCGAAACAGCCAAGCACA
TAGAAGAAGCCATTGATGAAGGTCATTCGTCCATTTGCACAATTGATCGTAAACATAGCGATGAGCAGAGAGATCAATCA
TTACATGGCGTTCCAACAAAGCGCGGGTATGATCGGGACGAATGGCCAATGGCGATGTGTAAAGAAGGGGGCACAGGAGC
TTCTGTCAAATATGTAAGCCCTTCAGATAATAGAGGCGCAGGCTCGTGGGTAAGTCATCAACTATCAGATGATCCTGACG
GCACAAGAATTCAATTTATCATCGATTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(46-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

71.698

74.648

0.535