Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PNF30_RS18705 Genome accession   NZ_CP116774
Coordinates   3660562..3661941 (-) Length   459 a.a.
NCBI ID   WP_025092231.1    Uniprot ID   A0AAP7QRW5
Organism   Bacillus safensis strain SRCM125915     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3655562..3666941
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PNF30_RS18685 (PNF30_18685) ispF 3657044..3657520 (-) 477 WP_003216928.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  PNF30_RS18690 (PNF30_18690) ispD 3657524..3658213 (-) 690 WP_024425583.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  PNF30_RS18695 (PNF30_18695) - 3658227..3659327 (-) 1101 WP_095407564.1 PIN/TRAM domain-containing protein -
  PNF30_RS18700 (PNF30_18700) disA 3659480..3660559 (-) 1080 WP_024425581.1 DNA integrity scanning diadenylate cyclase DisA -
  PNF30_RS18705 (PNF30_18705) radA 3660562..3661941 (-) 1380 WP_025092231.1 DNA repair protein RadA Machinery gene
  PNF30_RS18710 (PNF30_18710) clpC 3662035..3664470 (-) 2436 WP_048240170.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  PNF30_RS18715 (PNF30_18715) - 3664467..3665558 (-) 1092 WP_058838237.1 protein arginine kinase -
  PNF30_RS18720 (PNF30_18720) - 3665563..3666120 (-) 558 WP_205183538.1 UvrB/UvrC motif-containing protein -
  PNF30_RS18725 (PNF30_18725) - 3666135..3666599 (-) 465 WP_003217184.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50009.49 Da        Isoelectric Point: 7.3829

>NTDB_id=780206 PNF30_RS18705 WP_025092231.1 3660562..3661941(-) (radA) [Bacillus safensis strain SRCM125915]
MAKTKSKFICQSCGYESAKWMGKCPGCGTWNSMTEEVVRKEPANRRSAFNHSVQTIQKPSPISAIETSEEPRIKTNLEEF
NRVLGSGIVKGSLVLIGGDPGIGKSTLLLQVSAQLSDKNQNVLYISGEESIKQTKLRADRLGIESASLHVLAETDMEYIT
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAQLMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNELGIFEMREEGLTEVLNPSEIFLEERSAGVSGSCVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFKDAAPHQADCFIGEVGLT
GEVRRVSRIEQRVQEAAKLGFKRMFIPQANIDGWKKPRGIELVGVENVAEALRISLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=780206 PNF30_RS18705 WP_025092231.1 3660562..3661941(-) (radA) [Bacillus safensis strain SRCM125915]
ATGGCTAAGACAAAATCAAAATTTATATGCCAATCGTGCGGTTATGAGTCAGCCAAATGGATGGGGAAATGCCCAGGCTG
CGGCACGTGGAACAGTATGACAGAAGAGGTCGTTCGCAAAGAGCCGGCAAACCGTCGAAGTGCTTTTAATCATTCTGTTC
AAACCATTCAAAAACCTTCACCTATATCTGCAATTGAAACATCCGAAGAACCCCGAATTAAAACGAATTTAGAAGAATTT
AACCGAGTATTAGGAAGTGGAATTGTCAAAGGCTCTCTTGTTCTCATTGGCGGAGATCCTGGTATTGGGAAGTCCACATT
ATTATTACAAGTATCAGCACAGCTCTCAGACAAAAATCAGAATGTGTTATACATATCTGGTGAGGAGTCCATTAAACAAA
CGAAGCTAAGAGCGGACCGCCTTGGCATTGAAAGCGCCTCTTTACATGTTTTGGCTGAAACCGATATGGAGTATATAACG
TCTGCTATACAGGAGATGAAACCTGCTTTTGTTGTGGTGGATTCGATTCAAACCGTTTATCAAAGCGATATTACGTCAGC
TCCCGGTTCTGTATCTCAAGTAAGAGAATGTACAGCACAGCTGATGAAAATTGCCAAGACAAATGGTATTCCTATTTTTA
TCGTTGGTCACGTGACCAAAGAAGGCTCAATCGCAGGTCCACGCCTTTTAGAGCATATGGTGGACACAGTTCTTTATTTT
GAAGGCGAGCGTCATCATACGTTTCGTATCTTACGGGCGGTCAAAAACCGATTTGGTTCAACGAATGAATTGGGAATTTT
TGAAATGAGAGAGGAAGGGCTCACGGAAGTATTAAACCCATCAGAAATCTTTTTAGAAGAGCGGTCGGCAGGTGTATCTG
GTTCGTGTGTTGTTGCCTCAATGGAAGGAACAAGACCCGTACTTGTCGAGATACAGGCGTTAATTTCCCCGACAAGCTTT
GGGAATCCGCGAAGAATGGCCACTGGCCTTGATCATAATCGAGTGTCGCTCCTCATGGCAGTTTTAGAAAAACGCGTGGG
GCTGCTGCTGCAAAACCAAGATGCGTATTTAAAGGTCGCAGGCGGTGTGAAGCTTGACGAACCGGCGATTGACTTAGCCA
TTGCCGTCAGTATTGCATCAAGCTTTAAAGACGCAGCGCCGCATCAAGCGGATTGCTTTATAGGAGAGGTCGGTCTGACG
GGAGAAGTCAGAAGAGTATCAAGAATTGAACAACGAGTGCAGGAAGCTGCGAAACTAGGATTCAAGCGAATGTTTATTCC
TCAAGCGAATATAGATGGATGGAAAAAGCCGAGAGGGATTGAGTTAGTTGGTGTAGAAAATGTAGCGGAGGCACTTCGAA
TTTCACTAGGGGGATCATAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(67-222)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

87.336

99.782

0.871
  radA Streptococcus mitis NCTC 12261

61.589

98.693

0.608
  radA Streptococcus pneumoniae Rx1

61.589

98.693

0.608
  radA Streptococcus pneumoniae D39

61.589

98.693

0.608
  radA Streptococcus pneumoniae R6

61.589

98.693

0.608
  radA Streptococcus pneumoniae TIGR4

61.589

98.693

0.608
  radA Streptococcus mitis SK321

61.589

98.693

0.608