Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   PNF30_RS02755 Genome accession   NZ_CP116774
Coordinates   566327..567724 (+) Length   465 a.a.
NCBI ID   WP_272510693.1    Uniprot ID   -
Organism   Bacillus safensis strain SRCM125915     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 561327..572724
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PNF30_RS02735 (PNF30_02735) - 562329..562997 (-) 669 WP_272510692.1 YigZ family protein -
  PNF30_RS02740 (PNF30_02740) degS 563217..564392 (+) 1176 WP_003213166.1 sensor histidine kinase Regulator
  PNF30_RS02745 (PNF30_02745) degU 564457..565146 (+) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  PNF30_RS02750 (PNF30_02750) - 565364..566206 (+) 843 WP_095409677.1 DegV family protein -
  PNF30_RS02755 (PNF30_02755) comFA 566327..567724 (+) 1398 WP_272510693.1 DEAD/DEAH box helicase Machinery gene
  PNF30_RS02760 (PNF30_02760) - 567774..568067 (+) 294 WP_041088040.1 late competence development ComFB family protein -
  PNF30_RS02765 (PNF30_02765) comFC 568064..568750 (+) 687 WP_263639812.1 ComF family protein Machinery gene
  PNF30_RS02770 (PNF30_02770) - 568807..569226 (+) 420 WP_039181808.1 TIGR03826 family flagellar region protein -
  PNF30_RS02775 (PNF30_02775) flgM 569306..569569 (+) 264 WP_095409676.1 flagellar biosynthesis anti-sigma factor FlgM -
  PNF30_RS02780 (PNF30_02780) - 569588..570070 (+) 483 WP_144472023.1 flagellar protein FlgN -
  PNF30_RS02785 (PNF30_02785) flgK 570086..571606 (+) 1521 WP_144483665.1 flagellar hook-associated protein FlgK -
  PNF30_RS02790 (PNF30_02790) flgL 571616..572524 (+) 909 WP_144472025.1 flagellar hook-associated protein FlgL -

Sequence

Protein


Download         Length: 465 a.a.        Molecular weight: 53260.15 Da        Isoelectric Point: 10.0383

>NTDB_id=780158 PNF30_RS02755 WP_272510693.1 566327..567724(+) (comFA) [Bacillus safensis strain SRCM125915]
MNMDKAMELKRQLYSRHLLTVETRCSQSNLDWLEEKGLVNRTPAIERKADGFTCYRCGVSHKRYFAHSPCEECQKDCMYC
RSCIMMGKATECGFLYEWTGPKMVETYRAELTWQGELSKGQKRASERMIEAIKNKFDLLVWAVCGAGKTEVLFHGIEYAL
NQGMSVCIATPRTDVVLELEPRLRKAFQGIRIAVLYGGSSQRFQIAPLVIATTHQLMRYKNAFDVLIVDEVDAFPYSMDE
RLQFAVLKAMRKNGGVRIYLSATPSKKMTREVSRGKLEAIKIPLRFHQQPLPVPTFQWIGHWKKKLKKKQLPSKVMNWMQ
RHIANKRRVLLFVPSIAVMKKATKILREHSLNVEGVSADDPVRKQKVQHFRDYAYDVLVTTTILERGVTIPNVQVGVLGS
ESTIFTESALVQISGRVGRHPDYCTGDVFLFHFGLTRSMKQAKNHIVKMNDTAAKEFTEKQCGFN

Nucleotide


Download         Length: 1398 bp        

>NTDB_id=780158 PNF30_RS02755 WP_272510693.1 566327..567724(+) (comFA) [Bacillus safensis strain SRCM125915]
TTGAACATGGACAAAGCAATGGAACTAAAGCGGCAGCTGTATTCACGCCATCTGCTCACTGTCGAAACAAGGTGCTCACA
GTCCAATTTAGATTGGCTGGAGGAGAAAGGCTTAGTTAATCGAACACCTGCCATTGAGAGGAAAGCAGATGGTTTTACAT
GCTACCGGTGTGGTGTATCACACAAGCGGTACTTTGCTCACTCTCCATGTGAAGAGTGTCAAAAGGATTGTATGTACTGT
AGATCATGCATCATGATGGGAAAAGCAACTGAATGTGGATTTCTTTATGAATGGACGGGTCCAAAAATGGTAGAAACATA
TCGAGCCGAATTAACATGGCAGGGAGAGCTGTCTAAAGGACAAAAAAGAGCATCAGAAAGAATGATCGAAGCTATTAAAA
ACAAATTTGATCTACTCGTCTGGGCGGTTTGCGGAGCAGGGAAAACGGAAGTGCTTTTTCATGGAATTGAATATGCGTTA
AATCAAGGAATGAGTGTCTGTATTGCGACACCTAGAACAGATGTTGTGCTTGAACTCGAACCACGGCTTAGAAAAGCATT
TCAAGGGATAAGAATAGCCGTTCTTTATGGAGGCAGTTCACAAAGGTTTCAAATAGCGCCGCTTGTGATCGCCACAACCC
ACCAGCTGATGAGATACAAAAATGCATTTGATGTGCTCATTGTAGATGAAGTCGATGCCTTCCCTTATTCAATGGATGAA
CGTCTCCAATTTGCTGTTCTAAAGGCGATGAGAAAGAACGGAGGGGTTAGGATATATTTAAGTGCGACACCCTCTAAAAA
AATGACGAGAGAGGTTTCTCGTGGAAAACTGGAAGCGATAAAAATTCCTCTGCGTTTTCATCAACAACCATTACCCGTCC
CGACCTTTCAATGGATCGGACATTGGAAAAAGAAATTAAAAAAGAAGCAGCTTCCTTCTAAAGTGATGAATTGGATGCAG
AGACATATTGCCAATAAGAGAAGAGTATTACTTTTTGTTCCTTCTATTGCTGTCATGAAGAAGGCAACGAAGATTCTTCG
AGAACACTCCTTAAACGTAGAGGGAGTATCTGCCGATGATCCAGTTAGGAAACAAAAGGTTCAGCACTTTAGAGATTACG
CATACGATGTACTAGTTACAACCACTATTTTAGAAAGAGGCGTAACCATACCAAATGTTCAAGTAGGGGTACTAGGTTCG
GAATCTACTATTTTTACAGAGAGTGCACTTGTTCAGATTTCTGGAAGAGTGGGCAGACATCCCGATTATTGTACAGGAGA
TGTTTTCCTTTTTCATTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAATCATATCGTAAAAATGAATGATACGGCTG
CAAAAGAGTTTACTGAAAAACAGTGTGGCTTCAACTAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(116-265)

Helicase conserved C-terminal domain (Helicase_C)

(324-420)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

52.193

98.065

0.512