Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   PNF29_RS08495 Genome accession   NZ_CP116773
Coordinates   1615848..1616465 (+) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis strain SRCM125727     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1610848..1621465
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PNF29_RS08460 (PNF29_08460) aroE 1611506..1612348 (+) 843 WP_041351695.1 shikimate dehydrogenase -
  PNF29_RS08465 (PNF29_08465) yhbY 1612342..1612632 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  PNF29_RS08470 (PNF29_08470) nadD 1612644..1613213 (+) 570 WP_041333756.1 nicotinate-nucleotide adenylyltransferase -
  PNF29_RS08475 (PNF29_08475) yqeK 1613203..1613763 (+) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  PNF29_RS08480 (PNF29_08480) rsfS 1613781..1614137 (+) 357 WP_014480315.1 ribosome silencing factor -
  PNF29_RS08485 (PNF29_08485) yqeM 1614134..1614877 (+) 744 WP_029317885.1 class I SAM-dependent DNA methyltransferase -
  PNF29_RS08490 (PNF29_08490) comER 1614943..1615764 (-) 822 WP_014480313.1 late competence protein ComER -
  PNF29_RS08495 (PNF29_08495) comEA 1615848..1616465 (+) 618 WP_004398514.1 competence protein ComEA Machinery gene
  PNF29_RS08500 (PNF29_08500) comEB 1616532..1617101 (+) 570 WP_003229978.1 ComE operon protein 2 -
  PNF29_RS08505 (PNF29_08505) comEC 1617105..1619435 (+) 2331 WP_144453705.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  PNF29_RS08510 (PNF29_08510) yqzM 1619475..1619609 (-) 135 WP_003229983.1 YqzM family protein -
  PNF29_RS08515 (PNF29_08515) - 1619650..1619799 (+) 150 WP_120363468.1 hypothetical protein -
  PNF29_RS08520 (PNF29_08520) holA 1619839..1620882 (+) 1044 WP_041333763.1 DNA polymerase III subunit delta -
  PNF29_RS08525 (PNF29_08525) rpsT 1620897..1621163 (-) 267 WP_003229989.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=780099 PNF29_RS08495 WP_004398514.1 1615848..1616465(+) (comEA) [Bacillus subtilis strain SRCM125727]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=780099 PNF29_RS08495 WP_004398514.1 1615848..1616465(+) (comEA) [Bacillus subtilis strain SRCM125727]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TACAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39