Detailed information NTG0779724

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PMJ85_RS02185
• Genome accession: NZ_CP116718
• Coordinates: 474348..475496 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain 2858
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 469348..480496

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PMJ85_RS02160 (PMJ85_02160)	-	470499..470795 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PMJ85_RS02165 (PMJ85_02165)	-	470792..471385 (-)	594	WP_003084544.1	YggT family protein	-
PMJ85_RS02170 (PMJ85_02170)	proC	471396..472217 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PMJ85_RS02175 (PMJ85_02175)	-	472229..472921 (-)	693	WP_015649764.1	YggS family pyridoxal phosphate-dependent enzyme	-
PMJ85_RS02180 (PMJ85_02180)	pilT	473136..474170 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PMJ85_RS02185 (PMJ85_02185)	pilU	474348..475496 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PMJ85_RS02190 (PMJ85_02190)	czcD	475504..476403 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PMJ85_RS02195 (PMJ85_02195)	-	476557..476961 (+)	405	WP_003084560.1	NINE protein	-
PMJ85_RS02200 (PMJ85_02200)	-	477204..478577 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
PMJ85_RS02205 (PMJ85_02205)	-	478574..479758 (+)	1185	WP_015649763.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=779724 PMJ85_RS02185 WP_003100959.1 474348..475496(+) (pilU) [Pseudomonas aeruginosa strain 2858]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=779724 PMJ85_RS02185 WP_003100959.1 474348..475496(+) (pilU) [Pseudomonas aeruginosa strain 2858]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382