Detailed information NTG0779634

Bioinformatically predicted

Overview

• Name: comEC/celB
• Type: Machinery gene
• Locus tag: POF57_RS04640
• Genome accession: NZ_CP116714
• Coordinates: 855870..858107 (+)
• Length: 745 a.a.
• NCBI ID: WP_000939900.1
• Uniprot ID: -
• Organism: Streptococcus agalactiae strain A909 Cas9 AEKO
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 850870..863107

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
POF57_RS04615 (POF57_04615)	-	851464..853050 (+)	1587	WP_000673089.1	DEAD/DEAH box helicase	-
POF57_RS04620 (POF57_04620)	-	853235..853501 (-)	267	WP_000598736.1	GIY-YIG nuclease family protein	-
POF57_RS04625 (POF57_04625)	-	853494..854258 (-)	765	WP_000567423.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
POF57_RS04630 (POF57_04630)	-	854393..855133 (+)	741	WP_000500220.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
POF57_RS04635 (POF57_04635)	-	855233..855886 (+)	654	WP_000461745.1	helix-hairpin-helix domain-containing protein	-
POF57_RS04640 (POF57_04640)	comEC/celB	855870..858107 (+)	2238	WP_000939900.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
POF57_RS04645 (POF57_04645)	-	858233..859042 (+)	810	WP_000153219.1	Cof-type HAD-IIB family hydrolase	-
POF57_RS04650 (POF57_04650)	-	859053..859997 (+)	945	WP_000200828.1	LacI family DNA-binding transcriptional regulator	-
POF57_RS04655 (POF57_04655)	-	860056..861048 (-)	993	WP_000801001.1	alpha/beta hydrolase	-
POF57_RS04660 (POF57_04660)	-	861224..861952 (+)	729	WP_000468964.1	methyltransferase domain-containing protein	-
POF57_RS04665 (POF57_04665)	holA	862006..863043 (+)	1038	WP_000560292.1	DNA polymerase III subunit delta	-

Sequence

Protein

• Length: 745 a.a.
• Molecular weight: 85441.31 Da
• Isoelectric Point: 9.7742

>NTDB_id=779634 POF57_RS04640 WP_000939900.1 855870..858107(+) (comEC/celB) [Streptococcus agalactiae strain A909 Cas9 AEKO]
MLQLTKYFPLKPIYLALLVFQIYLLVFSWTMLGCAFLLFSFIFLIYQYDRETIFKTIAIVIFFLFYFLWQNHNMNVQYQR
VPNHISQIKVRIDTISINGDVLSFQADASGNTYQAFYTLKNKSEKDYFQNLDNNIMIIADIKLEEAEERRHFNGFDYRQY
LKRHGIYRIAKVTKIKQIRLFQHRSFFALMSKWRRSAIVISQTFPNPMRHYMSGLLFGYLDKTFDDMSDLYSSLGIIHLF
ALSGMQVGFFLGIFRYICLRIGLRLDHVWLLQIPFSLIYAGLTGFSISVVRALIQSLLSHSGVKKDENFALCLLICLISL
PHSLLTTGGVLSFAYAFILTMTSFDHFSSIKKVAIESLTVSVGILPILTYYFSGFQPISIILTALLSFAFDIIFLPLLTV
IFVLSPIVKLSCINSLFEILEVLLKWTGQLFPRPLIFGKPSLFLLIVMIIILGLLYDYYHSKCFRYCSLLIIFTLFFITK
NPITNEVAILDVGQGDSILVRDWLGKTILIDTGGRVRFEQPEEWKQKVNQSNAERTLIPYLKSRGISKIDDLVITHTDTD
HMGDMEVISKHFKVARLITSSGSLTNSQYVKHLSKIGVAVKSIEAGDKLAVMGSYLQVLYPWHKGDGKNNDSIVLYGHLL
GKGFLFTGDLEEEGEKQLLEAYPNLSVDILKAGHHGSKGSSSLSFLKKLSPSVVLVSAGKNNRYQHPHQETLQRFQKIKS
KIFRTDQSGTIRLTGWWKWHIQTVR

Nucleotide

• Length: 2238 bp

>NTDB_id=779634 POF57_RS04640 WP_000939900.1 855870..858107(+) (comEC/celB) [Streptococcus agalactiae strain A909 Cas9 AEKO]
ATGTTACAATTGACTAAGTATTTTCCTCTAAAACCTATTTATTTAGCATTGTTGGTCTTCCAAATTTACTTACTAGTGTT
TTCTTGGACAATGCTTGGTTGTGCCTTTCTTTTATTTTCTTTTATTTTTCTGATTTATCAATATGATCGTGAAACTATTT
TTAAAACAATAGCAATAGTAATTTTTTTCTTATTTTATTTTTTATGGCAAAATCACAATATGAATGTCCAATATCAAAGA
GTACCGAATCATATTAGCCAGATTAAAGTGCGTATTGATACTATTTCTATCAATGGTGATGTTTTATCATTCCAGGCAGA
TGCTTCAGGTAACACTTATCAAGCTTTTTACACATTAAAAAATAAAAGTGAGAAAGATTATTTTCAAAATCTTGATAATA
ATATAATGATAATTGCAGATATCAAACTTGAAGAAGCAGAGGAGAGAAGGCATTTTAATGGCTTTGATTATCGTCAGTAT
TTAAAAAGACATGGAATTTATCGTATCGCCAAAGTGACAAAGATAAAACAGATACGCTTATTTCAACATAGGTCTTTCTT
TGCTCTTATGTCTAAGTGGCGTAGAAGTGCAATTGTTATTAGTCAAACTTTTCCAAATCCTATGCGTCACTATATGTCAG
GGCTTTTGTTTGGATATCTAGATAAGACCTTTGATGACATGTCCGATTTATATAGTAGTCTAGGTATTATACATTTATTT
GCTTTGTCAGGTATGCAAGTAGGTTTTTTTCTCGGTATTTTTCGTTATATCTGTCTACGTATTGGCTTACGTCTAGACCA
TGTTTGGTTACTTCAAATACCATTCTCGCTAATTTATGCTGGTTTAACAGGCTTTAGTATCTCAGTCGTTAGGGCACTTA
TTCAATCTTTATTATCACATAGCGGTGTCAAGAAAGATGAGAACTTTGCTCTCTGCTTGTTAATTTGTCTTATCTCCCTC
CCCCACTCACTTTTGACTACGGGAGGAGTTCTTAGCTTTGCTTATGCTTTTATACTTACGATGACCTCCTTTGATCATTT
TTCGAGTATAAAAAAAGTAGCTATCGAATCTTTGACAGTCTCTGTAGGAATTCTTCCCATACTAACCTACTATTTTTCGG
GTTTTCAACCAATATCAATTATATTAACAGCACTTTTATCTTTTGCATTTGATATTATATTTTTGCCTTTATTAACTGTT
ATATTTGTCTTATCGCCTATCGTTAAATTAAGTTGTATTAATAGTTTGTTTGAAATCCTAGAAGTGTTATTAAAATGGAC
TGGGCAACTGTTTCCAAGGCCACTTATTTTTGGAAAGCCCAGCCTTTTTCTTTTAATAGTCATGATTATAATTTTGGGAT
TACTTTATGATTATTATCATTCTAAATGTTTTCGTTATTGCTCCCTTCTTATTATCTTTACCTTGTTTTTTATCACTAAG
AATCCAATTACTAACGAGGTTGCGATTTTAGATGTTGGACAGGGAGATAGTATTTTAGTGAGGGATTGGTTAGGAAAAAC
AATTTTAATTGATACTGGGGGAAGGGTGAGATTTGAACAGCCTGAAGAATGGAAACAAAAAGTAAATCAGTCTAATGCTG
AGAGAACGCTCATTCCTTACTTGAAAAGCAGAGGTATTAGCAAGATAGATGATTTAGTGATAACTCATACCGATACAGAT
CATATGGGGGATATGGAAGTTATCTCAAAGCATTTTAAAGTTGCACGTTTGATTACAAGTTCAGGTTCTTTAACGAATTC
GCAGTACGTTAAGCATTTATCAAAGATAGGTGTAGCGGTAAAATCTATAGAAGCCGGTGATAAACTTGCTGTCATGGGAA
GTTATTTACAAGTACTTTACCCATGGCACAAGGGTGATGGAAAAAATAATGATTCAATTGTTTTATATGGACATTTATTA
GGAAAAGGCTTCTTATTTACCGGTGATTTGGAGGAAGAGGGAGAAAAGCAGTTATTAGAAGCTTATCCTAATTTATCAGT
AGATATCCTTAAAGCAGGACATCATGGTTCTAAGGGCTCATCAAGTCTATCCTTTCTGAAAAAGTTGTCTCCTAGTGTGG
TTCTAGTTTCAGCTGGTAAAAATAATCGTTACCAGCATCCTCATCAAGAGACTTTACAAAGGTTCCAAAAGATTAAAAGC
AAGATTTTCCGAACGGATCAATCAGGTACAATTAGGCTAACAGGATGGTGGAAGTGGCATATTCAGACAGTTCGTTGA

Domains

Predicted by InterproScan.

• Competence protein (Competence) (215-458)

• Domain of unknown function (DUF4131) (DUF4131) (27-177)

• Metallo-beta-lactamase superfamily (Lactamase_B) (491-576)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC/celB	Streptococcus mitis NCTC 12261	48.4	100	0.487
comEC/celB	Streptococcus mitis SK321	48.529	100	0.487
comEC/celB	Streptococcus pneumoniae TIGR4	47.059	100	0.472
comEC/celB	Streptococcus pneumoniae Rx1	46.586	100	0.467
comEC/celB	Streptococcus pneumoniae D39	46.586	100	0.467
comEC/celB	Streptococcus pneumoniae R6	46.586	100	0.467
comEC	Lactococcus lactis subsp. cremoris KW2	43.338	99.732	0.432