Detailed information NTG0779566

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: QY490_RS26220
• Genome accession: NZ_CP129520
• Coordinates: 5679671..5680735 (-)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: A0A0H2ZID6
• Organism: Pseudomonas aeruginosa strain LG03
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5674671..5685735

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QY490_RS26195 (QY490_26195)	aroK	5675206..5675724 (-)	519	WP_003095833.1	shikimate kinase AroK	-
QY490_RS26200 (QY490_26200)	pilQ	5675736..5677880 (-)	2145	WP_003114575.1	type 4a pilus secretin PilQ	Machinery gene
QY490_RS26205 (QY490_26205)	comL/pilP	5677934..5678458 (-)	525	WP_003095836.1	type 4a pilus biogenesis lipoprotein PilP	Machinery gene
QY490_RS26210 (QY490_26210)	pilO	5678455..5679078 (-)	624	WP_003103268.1	type 4a pilus biogenesis protein PilO	Machinery gene
QY490_RS26215 (QY490_26215)	pilN	5679075..5679671 (-)	597	WP_003095839.1	type 4a pilus biogenesis protein PilN	Machinery gene
QY490_RS26220 (QY490_26220)	pilM	5679671..5680735 (-)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
QY490_RS26225 (QY490_26225)	-	5680920..5683388 (+)	2469	WP_003114576.1	penicillin-binding protein 1A	-
QY490_RS26230 (QY490_26230)	-	5683493..5684761 (-)	1269	WP_003103278.1	malic enzyme	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=779566 QY490_RS26220 WP_003110888.1 5679671..5680735(-) (pilM) [Pseudomonas aeruginosa strain LG03]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=779566 QY490_RS26220 WP_003110888.1 5679671..5680735(-) (pilM) [Pseudomonas aeruginosa strain LG03]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGCTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAAAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterProScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A0H2ZID6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM/pilM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466