Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   POF64_RS01140 Genome accession   NZ_CP116713
Coordinates   198074..199117 (+) Length   347 a.a.
NCBI ID   WP_223295889.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain A909 Cas9 AEKOrevertant     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 193074..204117
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF64_RS01130 (POF64_01130) - 196671..197036 (+) 366 WP_000285373.1 DUF1033 family protein -
  POF64_RS01135 (POF64_01135) comYA 197209..198180 (+) 972 WP_000250805.1 competence type IV pilus ATPase ComGA Machinery gene
  POF64_RS01140 (POF64_01140) comYB 198074..199117 (+) 1044 WP_223295889.1 competence type IV pilus assembly protein ComGB Machinery gene
  POF64_RS01145 (POF64_01145) comGC/cglC 199114..199443 (+) 330 WP_000793380.1 competence type IV pilus major pilin ComGC Machinery gene
  POF64_RS01150 (POF64_01150) comYD 199418..199831 (+) 414 WP_000244261.1 competence type IV pilus minor pilin ComGD Machinery gene
  POF64_RS01155 (POF64_01155) comGE 199803..200102 (+) 300 WP_000234804.1 competence type IV pilus minor pilin ComGE -
  POF64_RS01160 (POF64_01160) comGF 200056..200517 (+) 462 WP_001874812.1 competence type IV pilus minor pilin ComGF -
  POF64_RS01165 (POF64_01165) comGG 200495..200866 (+) 372 WP_000601104.1 competence type IV pilus minor pilin ComGG -
  POF64_RS01170 (POF64_01170) comYH 200981..201955 (+) 975 WP_001008574.1 N-6 DNA methylase Machinery gene
  POF64_RS01175 (POF64_01175) - 201987..203180 (+) 1194 WP_000047534.1 acetate kinase -
  POF64_RS01180 (POF64_01180) - 203332..203538 (+) 207 WP_000798241.1 helix-turn-helix transcriptional regulator -
  POF64_RS01185 (POF64_01185) - 203597..203734 (+) 138 WP_001865900.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39936.65 Da        Isoelectric Point: 9.9002

>NTDB_id=779560 POF64_RS01140 WP_223295889.1 198074..199117(+) (comYB) [Streptococcus agalactiae strain A909 Cas9 AEKOrevertant]
MDKWISWLKKDISVRNRHKSKKLSLKKQRKVVQLFNNLFASGFSLTDMVTFLKRSKLLSDCYTDSMNKALLEGKDLSKML
GELGFSDTVITQVALADLHGNISRSLLKIESYLANLLLVRKKVIEVATYPLILLSFLVLIMIGLRNYLMPQLGENNFATR
LITNVPNIFLLLLAVVLIFSLIFYIIQKRLSRIKVACFLTTIPLVGSYVKLYLTAYYAREWGNLLSQGIELDQIVKVMQN
QKSKLFREIGYDMEEGFLSGKAFHQKVLDYPFFLTELSLMIEYGQVKAKLGTELDIYADEKWEDFFTKLARATQLIQPVI
FIFVALIIVMIYAAMLLPMYQNMEILS

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=779560 POF64_RS01140 WP_223295889.1 198074..199117(+) (comYB) [Streptococcus agalactiae strain A909 Cas9 AEKOrevertant]
ATAGACAAGTGGATATCTTGGCTGAAGAAGGACATATCAGTAAGAAACAGGCACAAGTCGAAAAAATTATCCCTCAAGAA
ACAACGGAAAGTAGTCCAACTTTTTAATAATCTTTTTGCTAGTGGTTTTTCTTTAACTGATATGGTCACCTTCTTAAAGA
GGAGTAAGTTATTGTCTGATTGTTATACAGATAGTATGAATAAGGCATTATTAGAGGGAAAAGATTTATCAAAAATGTTA
GGAGAGTTAGGTTTTTCTGACACTGTTATCACACAGGTTGCATTAGCTGATTTGCATGGTAACATTTCAAGGAGCCTACT
AAAGATTGAGTCTTATTTAGCTAATCTTTTGTTAGTTAGAAAAAAAGTAATAGAAGTAGCTACTTACCCATTGATATTAT
TGTCTTTTCTGGTGCTAATTATGATTGGCCTTAGGAATTATTTAATGCCCCAATTAGGAGAAAATAATTTTGCAACTAGA
CTGATTACAAATGTGCCGAATATTTTCTTATTACTTTTAGCAGTTGTACTTATTTTTAGTTTAATATTTTATATTATTCA
AAAGCGATTGTCGCGCATTAAAGTAGCTTGTTTTTTAACAACAATTCCCTTAGTTGGATCATATGTTAAGCTTTATTTAA
CTGCTTACTATGCCCGTGAGTGGGGAAATTTATTAAGTCAAGGTATTGAATTGGACCAAATTGTTAAAGTAATGCAAAAT
CAGAAATCCAAACTTTTTAGGGAAATAGGATATGACATGGAAGAAGGTTTTCTATCAGGTAAAGCATTTCACCAAAAAGT
ATTAGACTATCCGTTTTTCTTAACTGAGCTTAGTTTAATGATTGAATATGGCCAAGTTAAGGCGAAATTAGGAACAGAGT
TAGATATATATGCTGATGAGAAGTGGGAGGATTTTTTTACAAAATTAGCTAGAGCGACCCAGTTAATCCAACCCGTTATT
TTTATTTTTGTAGCTCTTATCATTGTTATGATTTATGCAGCAATGCTGTTACCAATGTATCAAAATATGGAGATATTATC
ATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(34-150)

Type II secretion system (T2SS), protein F (T2SSF)

(218-338)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

62.17

98.271

0.611
  comYB Streptococcus mutans UA159

61.877

98.271

0.608
  comYB Streptococcus gordonii str. Challis substr. CH1

56.305

98.271

0.553
  comGB/cglB Streptococcus mitis NCTC 12261

55.224

96.542

0.533
  comGB/cglB Streptococcus mitis SK321

54.627

96.542

0.527
  comGB/cglB Streptococcus pneumoniae Rx1

53.731

96.542

0.519
  comGB/cglB Streptococcus pneumoniae D39

53.731

96.542

0.519
  comGB/cglB Streptococcus pneumoniae R6

53.731

96.542

0.519
  comGB/cglB Streptococcus pneumoniae TIGR4

53.731

96.542

0.519
  comGB Lactococcus lactis subsp. cremoris KW2

50.148

97.118

0.487