Detailed information NTG0779477

Bioinformatically predicted

Overview

• Name: comEC/celB
• Type: Machinery gene
• Locus tag: POF58_RS04285
• Genome accession: NZ_CP116711
• Coordinates: 783506..785743 (+)
• Length: 745 a.a.
• NCBI ID: WP_000939909.1
• Uniprot ID: -
• Organism: Streptococcus agalactiae strain CNCTC 10/84 Cas9 AEKOrevertant
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 778506..790743

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
POF58_RS04260 (POF58_04260)	-	779101..780687 (+)	1587	WP_000673082.1	DEAD/DEAH box helicase	-
POF58_RS04265 (POF58_04265)	-	780872..781138 (-)	267	WP_000598736.1	GIY-YIG nuclease family protein	-
POF58_RS04270 (POF58_04270)	-	781131..781895 (-)	765	WP_000611114.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
POF58_RS04275 (POF58_04275)	-	782030..782770 (+)	741	WP_000500220.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
POF58_RS04280 (POF58_04280)	-	782869..783522 (+)	654	WP_000461742.1	helix-hairpin-helix domain-containing protein	-
POF58_RS04285 (POF58_04285)	comEC/celB	783506..785743 (+)	2238	WP_000939909.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
POF58_RS04290 (POF58_04290)	-	785869..786678 (+)	810	WP_000153219.1	Cof-type HAD-IIB family hydrolase	-
POF58_RS04295 (POF58_04295)	-	786689..787633 (+)	945	WP_000200830.1	LacI family DNA-binding transcriptional regulator	-
POF58_RS04300 (POF58_04300)	-	787692..788684 (-)	993	WP_000800993.1	alpha/beta hydrolase	-
POF58_RS04305 (POF58_04305)	-	788859..789587 (+)	729	WP_000468968.1	methyltransferase domain-containing protein	-
POF58_RS04310 (POF58_04310)	holA	789641..790678 (+)	1038	WP_000560295.1	DNA polymerase III subunit delta	-

Sequence

Protein

• Length: 745 a.a.
• Molecular weight: 85579.45 Da
• Isoelectric Point: 9.6538

>NTDB_id=779477 POF58_RS04285 WP_000939909.1 783506..785743(+) (comEC/celB) [Streptococcus agalactiae strain CNCTC 10/84 Cas9 AEKOrevertant]
MLQLTKYFPLKPIYLALLVFQIYLLVFSWTMLGCAFLLFTFIFLIYQYDRETIFKTIAIVIFFLFYFLWQNHNMNVQYQR
VPNHISQIEVRIDTISINGDVLSFQADASGNTYQAFYTLKNKSEKDYFQNLDNNIMIIADIKLEEAEERRHFNGFDYRQY
LKRHGIYRIAKVTKIKQIRLFQHRSFFDLMSKWRRSAIVISQTFPNPMRHYMSGLLFGYLDKTFDDMCDLYSSLGIIHLF
ALSGMQVGFFLGIFRYICLRIGLRLDHVWLLQIPFSLIYAGLTGFSISVVRALIQSLLSHSGVKKDENFALCLLICLISL
PHSLLTTGGVLSFAYAFILTMTSFDHFSSIKKLAIESLTVSVGILPILTYYFSGFQPISIILTALLSFAFDIIFLPLLTV
IFVLSPIVKLSCINSLFEILEVLLKWTGQLFPRPLIFGKPSLFLLIVMIIILGLLYDYYHSKCFRYCSLLIIFTLFFITK
NPITNEVAILDVGQGDSILVRDWLGKTILIDTGGRLRFEQPEEWKQKVNQSNAERTLIPYLKSRGISKIDDLVITHTDTD
HMGDMEVISKHFKVARLITSSGSLTNSQYVKRLSKIGVAVKSIEAGDKLSVMGSYLQVLYPWHKGDGKNNDSIVLYGHLL
GKGFLFTGDLEEEGEKQLLEAYPNLSVDILKAGHHGSKGSSSLSFLKKLSPSVVLVSAGKNNRYQHPHQETLQRFQKIKS
KIFRTDQSGTIRLTGWWKWHIQTVR

Nucleotide

• Length: 2238 bp

>NTDB_id=779477 POF58_RS04285 WP_000939909.1 783506..785743(+) (comEC/celB) [Streptococcus agalactiae strain CNCTC 10/84 Cas9 AEKOrevertant]
ATGTTACAATTGACTAAGTATTTTCCTCTAAAACCTATTTATTTAGCATTGTTGGTCTTCCAAATTTACTTACTAGTGTT
TTCTTGGACAATGCTTGGTTGTGCCTTTCTTTTATTTACTTTTATTTTTCTGATTTATCAATATGATCGTGAAACTATTT
TTAAAACAATAGCAATAGTAATTTTTTTCTTATTTTATTTTTTATGGCAAAATCACAATATGAATGTCCAATATCAAAGA
GTACCGAATCATATTAGCCAGATTGAAGTGCGTATTGATACTATTTCTATCAATGGTGATGTTTTATCATTCCAGGCAGA
TGCTTCAGGTAACACTTATCAAGCTTTTTACACATTAAAAAATAAAAGTGAGAAAGATTATTTTCAAAATCTTGATAATA
ATATAATGATAATTGCAGATATCAAACTTGAAGAAGCAGAGGAGAGAAGGCATTTTAATGGCTTTGATTATCGTCAGTAT
TTAAAAAGACATGGAATTTATCGTATCGCCAAAGTGACAAAGATAAAACAGATACGCTTATTTCAACATAGGTCTTTCTT
TGATCTTATGTCTAAGTGGCGTAGAAGTGCAATTGTTATTAGTCAAACTTTTCCAAATCCTATGCGTCACTATATGTCAG
GGCTTTTGTTTGGATATCTAGATAAGACCTTTGATGACATGTGCGATTTATATAGTAGTCTAGGTATTATACATTTATTT
GCTTTGTCAGGTATGCAAGTAGGTTTTTTTCTCGGTATTTTTCGTTATATCTGTCTACGTATTGGCTTACGCCTAGACCA
TGTTTGGTTACTTCAAATACCATTCTCGCTAATTTATGCTGGTTTAACAGGCTTTAGTATCTCAGTCGTTAGGGCACTTA
TTCAATCTTTATTATCACATAGCGGTGTCAAGAAAGATGAGAACTTTGCTCTCTGCTTGTTAATTTGTCTTATCTCTCTC
CCCCACTCACTTTTGACTACGGGAGGAGTTCTTAGCTTTGCTTATGCTTTTATACTTACGATGACCTCCTTTGATCATTT
TTCGAGTATAAAAAAACTAGCTATCGAATCTTTGACAGTCTCTGTAGGAATTCTTCCCATACTAACCTACTATTTTTCAG
GTTTTCAACCAATATCAATTATATTAACAGCACTTTTATCTTTTGCATTTGATATTATATTTTTGCCTTTATTAACTGTT
ATATTTGTCTTATCGCCTATCGTTAAATTAAGTTGTATTAATAGTTTGTTTGAAATCCTAGAAGTGTTATTAAAATGGAC
TGGGCAACTGTTTCCAAGGCCACTTATTTTTGGAAAGCCCAGCCTTTTTCTTTTAATAGTCATGATTATAATTTTGGGAT
TACTTTATGATTATTATCATTCTAAATGTTTTCGTTATTGCTCCCTTCTTATTATCTTTACCTTGTTTTTTATCACTAAG
AATCCAATTACTAACGAGGTCGCGATTTTAGATGTTGGACAAGGAGATAGTATTTTAGTGAGGGATTGGTTAGGAAAAAC
AATTTTAATTGATACTGGGGGAAGGTTGAGATTTGAACAGCCTGAAGAATGGAAACAAAAAGTAAATCAGTCTAATGCTG
AGAGAACGCTCATTCCTTACTTGAAAAGCAGAGGTATTAGCAAGATAGATGATTTAGTGATAACTCATACCGATACAGAT
CATATGGGGGATATGGAAGTTATCTCAAAGCATTTTAAAGTTGCACGTTTGATTACAAGTTCAGGTTCTTTAACGAATTC
GCAGTACGTTAAGCGTTTATCAAAGATAGGAGTAGCTGTAAAATCTATAGAAGCTGGTGATAAACTTTCTGTCATGGGAA
GTTATTTACAAGTACTTTACCCATGGCACAAGGGTGATGGAAAAAATAATGATTCAATTGTTTTATATGGACATTTATTA
GGAAAAGGCTTCTTATTTACCGGTGATTTGGAGGAAGAGGGAGAAAAGCAGTTATTAGAAGCTTATCCTAATTTATCAGT
AGATATCCTTAAAGCAGGACATCATGGTTCTAAGGGCTCATCAAGTCTATCCTTTCTGAAAAAGTTGTCTCCTAGTGTGG
TTCTAGTTTCAGCTGGTAAAAATAATCGTTACCAGCATCCTCATCAAGAGACTTTACAAAGGTTCCAAAAGATTAAAAGC
AAGATTTTCCGAACGGATCAATCAGGTACAATTAGGTTGACAGGATGGTGGAAGTGGCATATTCAGACAGTTCGTTGA

Domains

Predicted by InterproScan.

• Domain of unknown function (DUF4131) (DUF4131) (28-177)

• Competence protein (Competence) (215-458)

• Metallo-beta-lactamase superfamily (Lactamase_B) (491-576)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC/celB	Streptococcus mitis NCTC 12261	48.4	100	0.487
comEC/celB	Streptococcus mitis SK321	48.396	100	0.486
comEC/celB	Streptococcus pneumoniae TIGR4	46.925	100	0.471
comEC/celB	Streptococcus pneumoniae Rx1	46.452	100	0.466
comEC/celB	Streptococcus pneumoniae D39	46.452	100	0.466
comEC/celB	Streptococcus pneumoniae R6	46.452	100	0.466
comEC	Lactococcus lactis subsp. cremoris KW2	44.145	99.732	0.44