Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   POF56_RS01135 Genome accession   NZ_CP116710
Coordinates   197209..198180 (+) Length   323 a.a.
NCBI ID   WP_000250805.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain A909 dCas9     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 192209..203180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF56_RS01125 (POF56_01125) rpoC 192907..196557 (+) 3651 WP_000228729.1 DNA-directed RNA polymerase subunit beta' -
  POF56_RS01130 (POF56_01130) - 196671..197036 (+) 366 WP_000285373.1 DUF1033 family protein -
  POF56_RS01135 (POF56_01135) comYA 197209..198180 (+) 972 WP_000250805.1 competence type IV pilus ATPase ComGA Machinery gene
  POF56_RS01140 (POF56_01140) comYB 198074..199117 (+) 1044 WP_223295889.1 competence type IV pilus assembly protein ComGB Machinery gene
  POF56_RS01145 (POF56_01145) comGC/cglC 199114..199443 (+) 330 WP_000793380.1 competence type IV pilus major pilin ComGC Machinery gene
  POF56_RS01150 (POF56_01150) comYD 199418..199831 (+) 414 WP_000244261.1 competence type IV pilus minor pilin ComGD Machinery gene
  POF56_RS01155 (POF56_01155) comGE 199803..200102 (+) 300 WP_000234804.1 competence type IV pilus minor pilin ComGE -
  POF56_RS01160 (POF56_01160) comGF 200056..200517 (+) 462 WP_001874812.1 competence type IV pilus minor pilin ComGF -
  POF56_RS01165 (POF56_01165) comGG 200495..200866 (+) 372 WP_000601104.1 competence type IV pilus minor pilin ComGG -
  POF56_RS01170 (POF56_01170) comYH 200981..201955 (+) 975 WP_001008574.1 N-6 DNA methylase Machinery gene
  POF56_RS01175 (POF56_01175) - 201987..203180 (+) 1194 WP_000047534.1 acetate kinase -

Sequence

Protein


Download         Length: 323 a.a.        Molecular weight: 36801.32 Da        Isoelectric Point: 6.8469

>NTDB_id=779402 POF56_RS01135 WP_000250805.1 197209..198180(+) (comYA) [Streptococcus agalactiae strain A909 dCas9]
MVQSLAKQVIHQAVEVNAQDIYIIPKGDCYELYMRIDDERRFIDVFEFNRMASLISHFKFVAGMNVGEKRRSQLGSCDYE
LSEGRLVSLRLSSVGDYRGQESLVIRILYSGHQDLKYWFDNIKQMKEVLGIRGLYLFSGPVGSGKTTLMYQLASEVFKNK
QIITIEDPVEIKNDKMLQLQLNEDIGMTYDALIKLSLRHRPDILIIGEIRDQATARAVIRASLTGVMVFSTIHAKSIPGV
YDRLIELGVNYQELENSLKLIAYQRLIGGGSLIDFETGNFKKHSSDKWNRQVDILAEEGHISKKQAQVEKIIPQETTESS
PTF

Nucleotide


Download         Length: 972 bp        

>NTDB_id=779402 POF56_RS01135 WP_000250805.1 197209..198180(+) (comYA) [Streptococcus agalactiae strain A909 dCas9]
ATGGTTCAATCATTAGCAAAGCAAGTCATTCATCAGGCAGTAGAAGTAAATGCTCAAGATATTTATATCATTCCCAAAGG
TGATTGTTATGAACTCTATATGCGTATTGATGATGAAAGGCGGTTTATTGATGTTTTTGAGTTTAATAGGATGGCTAGTC
TTATTAGTCACTTTAAATTTGTGGCAGGCATGAACGTTGGAGAAAAAAGACGAAGTCAATTAGGTTCTTGTGACTATGAA
CTGTCAGAGGGAAGACTGGTTTCATTACGACTATCGAGTGTGGGAGATTATCGTGGTCAAGAATCTTTAGTTATTCGTAT
TTTGTATTCAGGTCATCAGGACTTAAAATATTGGTTTGATAATATAAAGCAAATGAAGGAAGTACTGGGTATAAGAGGGC
TATATCTTTTTTCCGGCCCTGTGGGGAGTGGTAAAACAACTCTCATGTATCAATTAGCTTCAGAAGTATTTAAAAATAAG
CAAATTATCACGATTGAAGATCCGGTAGAAATCAAGAATGACAAGATGTTACAACTCCAATTGAATGAGGATATTGGAAT
GACTTATGATGCTTTAATCAAACTGTCTTTACGGCATCGTCCAGATATTTTAATTATCGGAGAGATTAGAGATCAAGCGA
CGGCCCGTGCTGTTATTCGTGCAAGTTTAACGGGAGTGATGGTTTTTTCTACTATTCATGCTAAAAGTATTCCCGGAGTC
TATGATAGGCTTATAGAATTAGGGGTTAACTATCAAGAGTTAGAAAATAGTCTAAAATTAATAGCATATCAACGTTTAAT
TGGAGGAGGAAGCCTAATTGACTTTGAGACAGGTAATTTTAAAAAACACTCATCAGACAAGTGGAATAGACAAGTGGATA
TCTTGGCTGAAGAAGGACATATCAGTAAGAAACAGGCACAAGTCGAAAAAATTATCCCTCAAGAAACAACGGAAAGTAGT
CCAACTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

68.81

96.285

0.663
  comYA Streptococcus mutans UA140

68.81

96.285

0.663
  comYA Streptococcus gordonii str. Challis substr. CH1

63.636

98.762

0.628
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

62.701

96.285

0.604
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

61.736

96.285

0.594
  comGA/cglA/cilD Streptococcus pneumoniae D39

61.736

96.285

0.594
  comGA/cglA/cilD Streptococcus pneumoniae R6

61.736

96.285

0.594
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

61.736

96.285

0.594
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.935

95.975

0.594
  comGA Lactococcus lactis subsp. cremoris KW2

50.621

99.69

0.505