Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYH   Type   Machinery gene
Locus tag   POF63_RS01160 Genome accession   NZ_CP116708
Coordinates   193082..194056 (+) Length   324 a.a.
NCBI ID   WP_001008567.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain CNCTC 10/84 sCas9     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 188082..199056
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF63_RS01120 (POF63_01120) - 188773..189138 (+) 366 WP_000285373.1 DUF1033 family protein -
  POF63_RS01125 (POF63_01125) comYA 189310..190281 (+) 972 WP_000250801.1 competence type IV pilus ATPase ComGA Machinery gene
  POF63_RS01130 (POF63_01130) comYB 190175..191218 (+) 1044 WP_228313184.1 competence type IV pilus assembly protein ComGB Machinery gene
  POF63_RS01135 (POF63_01135) comGC/cglC 191215..191544 (+) 330 WP_000793380.1 competence type IV pilus major pilin ComGC Machinery gene
  POF63_RS01140 (POF63_01140) comYD 191519..191932 (+) 414 WP_000244259.1 competence type IV pilus minor pilin ComGD Machinery gene
  POF63_RS01145 (POF63_01145) comGE 191904..192203 (+) 300 WP_038406052.1 competence type IV pilus minor pilin ComGE -
  POF63_RS01150 (POF63_01150) comGF 192157..192618 (+) 462 WP_001874060.1 competence type IV pilus minor pilin ComGF -
  POF63_RS01155 (POF63_01155) comGG 192596..192967 (+) 372 WP_000601105.1 competence type IV pilus minor pilin ComGG -
  POF63_RS01160 (POF63_01160) comYH 193082..194056 (+) 975 WP_001008567.1 class I SAM-dependent methyltransferase Machinery gene
  POF63_RS01165 (POF63_01165) - 194088..195281 (+) 1194 WP_000047534.1 acetate kinase -
  POF63_RS01170 (POF63_01170) - 195409..195601 (+) 193 Protein_174 helix-turn-helix transcriptional regulator -
  POF63_RS01175 (POF63_01175) - 195580..195846 (+) 267 Protein_175 hypothetical protein -
  POF63_RS01180 (POF63_01180) - 195915..196580 (+) 666 WP_000008107.1 type II CAAX endopeptidase family protein -
  POF63_RS01185 (POF63_01185) proC 196601..197371 (-) 771 WP_001876660.1 pyrroline-5-carboxylate reductase -
  POF63_RS01190 (POF63_01190) pepA 197441..198508 (-) 1068 WP_001281322.1 glutamyl aminopeptidase -
  POF63_RS01195 (POF63_01195) - 198688..198927 (-) 240 WP_000660180.1 hypothetical protein -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 37117.05 Da        Isoelectric Point: 4.4041

>NTDB_id=779301 POF63_RS01160 WP_001008567.1 193082..194056(+) (comYH) [Streptococcus agalactiae strain CNCTC 10/84 sCas9]
MNFEKIETAYELILENIQTIENQLKTHIYDALIEQNSYYLGSSCDLDIVVVNNQKLRQLDLSQEEWRRTFQFIFIKSAQT
EQLQANHQFTPDSIGFILLFLLEELMSQETVDVLEIGSGTGNLAQTLLNNSSKELNYMGIEVDDLLIDLSASIAEIIGSS
AQFIQEDAVRPQILKESDVIISDLPVGYYPNDDIAKRYAVSSSKEHTYAHHLLMEQSLKYLKKDGIAIFLAPENLLTSPQ
SDLLKEWLKGYADVIAVLTLPETIFGSRQNAKSIFVLKKQAEQKPETFVYPLTDLQNRENMANFIENFQKWSRENSHYSK
NMIE

Nucleotide


Download         Length: 975 bp        

>NTDB_id=779301 POF63_RS01160 WP_001008567.1 193082..194056(+) (comYH) [Streptococcus agalactiae strain CNCTC 10/84 sCas9]
ATGAATTTTGAAAAAATTGAGACAGCCTATGAGCTGATTTTAGAAAATATCCAAACGATTGAGAACCAATTAAAAACTCA
TATTTATGATGCCTTAATTGAACAGAACTCTTATTACCTTGGTTCAAGTTGTGATTTAGATATTGTTGTGGTGAATAACC
AAAAATTACGTCAACTTGACTTAAGTCAAGAAGAATGGCGTCGCACTTTCCAGTTCATTTTTATCAAATCTGCGCAAACA
GAGCAATTACAAGCTAATCATCAGTTTACGCCAGATAGTATTGGTTTTATCTTGTTATTTCTTTTGGAAGAATTAATGAG
TCAAGAGACAGTTGATGTCTTGGAAATTGGAAGTGGAACTGGGAATTTAGCTCAGACTCTCCTCAATAACAGCTCGAAAG
AGTTAAATTATATGGGCATTGAAGTTGATGATCTTTTGATTGATCTATCAGCAAGCATTGCTGAAATTATAGGTTCTAGT
GCCCAATTTATCCAAGAGGATGCTGTTAGACCACAAATTTTGAAAGAAAGCGATGTAATCATTAGTGATTTACCAGTTGG
CTATTATCCTAATGATGATATTGCTAAACGATATGCTGTATCAAGTTCTAAAGAGCACACCTATGCTCACCATCTATTGA
TGGAGCAATCTCTTAAATATTTGAAAAAAGATGGAATCGCTATATTTTTAGCACCCGAAAACCTTTTAACAAGTCCACAA
AGTGATTTGCTGAAGGAGTGGTTAAAAGGATATGCAGATGTCATTGCCGTTTTAACTCTACCAGAAACTATTTTTGGAAG
TCGTCAAAATGCGAAATCTATATTTGTTCTCAAGAAGCAAGCAGAACAAAAACCAGAAACCTTTGTATATCCGCTGACAG
ATTTGCAAAATCGTGAGAATATGGCAAACTTCATTGAAAATTTTCAAAAATGGAGCAGAGAAAATAGTCATTACTCAAAA
AATATGATAGAATAG

Domains

Predicted by InterproScan.

N-6 DNA Methylase (N6_Mtase)

(70-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYH Streptococcus mutans UA159

66.984

97.222

0.651
  comYH Streptococcus mutans UA140

66.984

97.222

0.651