Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   PML25_RS05180 Genome accession   NZ_CP116672
Coordinates   1245224..1246282 (+) Length   352 a.a.
NCBI ID   WP_043909467.1    Uniprot ID   A0A0G8KJ77
Organism   Xanthomonas hortorum pv. pelargonii strain 305     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1240224..1251282
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PML25_RS05165 (PML25_05165) - 1240322..1241611 (+) 1290 WP_168958753.1 citrate synthase -
  PML25_RS05170 (PML25_05170) - 1241715..1242365 (-) 651 WP_168958754.1 hypothetical protein -
  PML25_RS05175 (PML25_05175) - 1242582..1245008 (-) 2427 WP_168958755.1 penicillin-binding protein 1A -
  PML25_RS05180 (PML25_05180) comM 1245224..1246282 (+) 1059 WP_043909467.1 pilus assembly protein PilM Machinery gene
  PML25_RS05185 (PML25_05185) - 1246282..1247049 (+) 768 WP_168958756.1 PilN domain-containing protein -
  PML25_RS05190 (PML25_05190) - 1247046..1247711 (+) 666 WP_006451354.1 type 4a pilus biogenesis protein PilO -
  PML25_RS05195 (PML25_05195) - 1247708..1248241 (+) 534 WP_168958757.1 pilus assembly protein PilP -
  PML25_RS05200 (PML25_05200) - 1248261..1250213 (+) 1953 WP_074058175.1 type IV pilus secretin PilQ family protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37624.24 Da        Isoelectric Point: 4.3882

>NTDB_id=778985 PML25_RS05180 WP_043909467.1 1245224..1246282(+) (comM) [Xanthomonas hortorum pv. pelargonii strain 305]
MGLLPKSQSPLIGVDISSTAVKLLQLSRSGNRFRVDHYAVEPLPPNAVVEKNIVEVEAVGEAIRRAINRSGSKAKNAAAA
VAGSAVITKLIPMPADLDDSDMEAQVELEATNYIPYPIEEVNLDFEVLGPMPNSPDMVQVLLAASRSENVELRQSALELG
GLVAKVMDVEAFAVENAFALVASELPVAADAVVALVDIGATMTTLSVLRSGRSLYSREQVFGGKQLTDEVMRRYGLTYEE
AGLAKRQGGLPESYEVEVLEPFKEATVQQISRLLQFFYAGSEFNRVDCIVLAGGCAALSRLPEMVEEQLGVTTVVANPLA
QMTLGPKVQAHALALDAPALMIATGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=778985 PML25_RS05180 WP_043909467.1 1245224..1246282(+) (comM) [Xanthomonas hortorum pv. pelargonii strain 305]
GTGGGGCTTCTACCCAAGAGTCAATCGCCACTGATTGGTGTCGATATCAGTTCCACTGCGGTCAAGCTCTTGCAGCTGTC
ACGTAGCGGGAATCGTTTTCGCGTGGATCATTACGCGGTGGAACCGTTGCCGCCGAACGCGGTCGTGGAAAAGAACATCG
TCGAAGTGGAGGCGGTGGGCGAAGCCATTCGCCGCGCCATCAATCGTTCCGGCAGCAAGGCCAAGAACGCGGCTGCGGCC
GTGGCCGGGTCGGCGGTGATCACCAAGCTGATCCCGATGCCGGCCGATCTGGACGACAGCGATATGGAAGCCCAGGTCGA
ACTCGAGGCCACCAATTACATTCCGTACCCGATCGAGGAAGTGAATCTCGATTTCGAGGTGCTTGGCCCGATGCCCAACA
GCCCGGACATGGTCCAGGTGCTGCTGGCTGCCTCGCGTTCGGAGAATGTGGAACTGCGCCAGTCCGCGCTGGAACTCGGT
GGCCTGGTTGCCAAGGTGATGGACGTAGAGGCATTTGCGGTCGAAAACGCGTTTGCCCTGGTCGCCAGCGAGCTGCCGGT
GGCCGCCGATGCAGTGGTTGCGCTGGTGGACATCGGCGCGACCATGACCACGCTGAGCGTGCTGCGTTCCGGTCGCAGTC
TGTATAGCCGCGAGCAGGTGTTCGGCGGCAAGCAGCTCACCGATGAAGTGATGCGCCGTTACGGCCTGACCTATGAAGAA
GCCGGCTTGGCCAAGCGCCAGGGCGGGTTGCCGGAGAGCTACGAGGTCGAAGTGCTGGAGCCGTTCAAAGAGGCGACGGT
GCAGCAGATCAGCCGCCTGTTGCAGTTCTTCTACGCGGGCAGCGAATTCAATCGCGTCGACTGCATCGTGCTGGCCGGCG
GTTGCGCCGCGCTGTCGCGCCTGCCGGAGATGGTGGAAGAGCAGCTCGGCGTGACCACCGTGGTCGCCAACCCGCTTGCA
CAGATGACGCTCGGCCCGAAGGTTCAGGCCCATGCGCTGGCGCTGGATGCGCCTGCATTGATGATTGCCACCGGCCTGGC
CCTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G8KJ77

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

50.852

100

0.509
  pilM Acinetobacter baumannii D1279779

50.568

100

0.506
  comM Acinetobacter baylyi ADP1

50.712

99.716

0.506
  pilM Legionella pneumophila strain ERS1305867

47.293

99.716

0.472