Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   PHA78_RS02365 Genome accession   NZ_CP116604
Coordinates   477958..479250 (+) Length   430 a.a.
NCBI ID   WP_024377223.1    Uniprot ID   -
Organism   Streptococcus sp. HN38     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 472958..484250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PHA78_RS02340 - 473763..474404 (+) 642 WP_024398623.1 response regulator transcription factor -
  PHA78_RS02345 - 474454..475854 (+) 1401 WP_029173958.1 Cof-type HAD-IIB family hydrolase -
  PHA78_RS02350 - 475856..476227 (+) 372 WP_002937017.1 S1 RNA-binding domain-containing protein -
  PHA78_RS02355 cysK 476254..477180 (-) 927 WP_029173960.1 cysteine synthase A -
  PHA78_RS02360 - 477269..477901 (-) 633 WP_272158415.1 YigZ family protein -
  PHA78_RS02365 comFA/cflA 477958..479250 (+) 1293 WP_024377223.1 DEAD/DEAH box helicase Machinery gene
  PHA78_RS02370 comFC/cflB 479243..479908 (+) 666 WP_024377224.1 ComF family protein Machinery gene
  PHA78_RS02375 raiA 479986..480528 (+) 543 WP_014637536.1 ribosome-associated translation inhibitor RaiA -

Sequence

Protein


Download         Length: 430 a.a.        Molecular weight: 48994.55 Da        Isoelectric Point: 9.1584

>NTDB_id=778757 PHA78_RS02365 WP_024377223.1 477958..479250(+) (comFA/cflA) [Streptococcus sp. HN38]
MKELENYYGRLFTKYQLTAKEREKAEKVPSITKKNNCFRCGTTFEEENKLPNDAYYCRACLLLGRVRSDEKLYHFPQKDF
SITKCLKWKGQLTDWQQRISDGLVANVENNRATLVHAVTGAGKTEMIYHTVASVIDKGGAVCLASPRIDVCIELYKRLQN
DFSVPISLLHGESEPYFRTPLVVATTHQLLKFYQAFDLVLIDEVDAFPYADNPMLYRAADNAVKEDGVQVFLTATSTDEL
DKKVRMGKLSRLSLPRRFHGNPLVVPQKVWFSKFDDTLKKNRLVPKLKKAIEEQRKSGFPLLIFVPEISKGQEFTKIMKK
TFPEETIGFVSSQTENRLEIVEGFRKREITVLISTTILERGVTFPCVDVFVVQANHYLYTASSLVQIAGRVGRSMERPTG
LLQFYHEGSTGAIEKAIAEIKQMNKEAGYV

Nucleotide


Download         Length: 1293 bp        

>NTDB_id=778757 PHA78_RS02365 WP_024377223.1 477958..479250(+) (comFA/cflA) [Streptococcus sp. HN38]
ATGAAAGAATTAGAAAATTATTATGGAAGATTATTTACCAAATACCAATTGACAGCAAAAGAAAGAGAAAAAGCAGAAAA
AGTGCCAAGTATTACAAAAAAGAATAACTGCTTTCGCTGTGGAACAACTTTTGAAGAAGAAAACAAATTGCCAAACGATG
CTTATTACTGTCGAGCCTGCTTGCTTCTAGGCAGAGTACGGTCAGACGAAAAACTCTATCATTTTCCTCAGAAAGATTTT
TCAATCACTAAGTGTTTAAAGTGGAAAGGTCAATTAACTGATTGGCAACAAAGAATTTCAGATGGACTAGTTGCAAACGT
GGAAAATAATCGTGCGACATTGGTTCATGCAGTAACAGGAGCAGGTAAGACAGAAATGATCTACCACACCGTTGCCTCAG
TGATTGATAAAGGCGGAGCGGTTTGCCTAGCCAGTCCTCGAATTGATGTTTGTATCGAACTCTATAAACGTCTGCAAAAT
GACTTTTCAGTTCCAATTAGTTTACTGCATGGAGAGTCTGAACCCTATTTCCGAACTCCATTAGTTGTAGCAACCACACA
TCAGTTATTAAAATTTTATCAGGCCTTTGATTTGGTTTTGATTGATGAAGTAGACGCCTTTCCCTATGCAGATAATCCCA
TGCTCTATCGAGCAGCAGATAATGCGGTCAAGGAAGACGGAGTTCAAGTTTTTCTGACGGCGACTTCAACAGATGAATTG
GATAAAAAAGTCAGAATGGGTAAATTAAGCCGTCTTAGTTTGCCAAGGCGCTTTCATGGCAACCCACTTGTTGTCCCGCA
AAAAGTCTGGTTTAGTAAATTCGATGATACCCTAAAGAAAAATAGACTAGTTCCAAAGTTGAAAAAAGCGATTGAAGAAC
AGAGAAAGTCGGGCTTTCCCTTACTCATTTTTGTCCCAGAAATCTCCAAAGGTCAAGAATTTACCAAGATAATGAAAAAA
ACATTCCCAGAAGAAACAATTGGCTTTGTATCCAGTCAAACAGAAAATCGCCTTGAAATAGTTGAAGGGTTTCGCAAGAG
AGAAATCACAGTCTTAATCTCGACTACTATTCTTGAACGTGGGGTGACCTTCCCATGTGTAGACGTCTTTGTTGTTCAAG
CCAATCATTACCTCTACACAGCGTCAAGTCTTGTTCAGATTGCAGGCCGGGTCGGCAGGAGTATGGAACGTCCGACTGGT
TTACTCCAGTTTTATCATGAGGGAAGTACAGGAGCCATTGAAAAGGCAATCGCTGAAATTAAACAGATGAACAAGGAGGC
TGGTTATGTCTAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(91-235)

Helicase conserved C-terminal domain (Helicase_C)

(289-393)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

67.053

100

0.672
  comFA/cflA Streptococcus pneumoniae Rx1

66.357

100

0.665
  comFA/cflA Streptococcus pneumoniae D39

66.357

100

0.665
  comFA/cflA Streptococcus pneumoniae R6

66.357

100

0.665
  comFA/cflA Streptococcus pneumoniae TIGR4

66.357

100

0.665
  comFA/cflA Streptococcus mitis SK321

66.125

100

0.663
  comFA Lactococcus lactis subsp. cremoris KW2

51.537

98.372

0.507
  comFA Latilactobacillus sakei subsp. sakei 23K

38.051

100

0.381
  comFA Bacillus subtilis subsp. subtilis str. 168

37.831

96.512

0.365