Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   QYH60_RS03555 Genome accession   NZ_CP129292
Coordinates   708647..709720 (+) Length   357 a.a.
NCBI ID   WP_081042697.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain KMGR2-43     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 703647..714720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QYH60_RS03550 (QYH60_03550) comGA 707815..708753 (+) 939 Protein_657 competence type IV pilus ATPase ComGA -
  QYH60_RS03555 (QYH60_03555) comGB 708647..709720 (+) 1074 WP_081042697.1 competence type IV pilus assembly protein ComGB Machinery gene
  QYH60_RS03560 (QYH60_03560) - 709734..709874 (+) 141 WP_023164653.1 hypothetical protein -
  QYH60_RS03565 (QYH60_03565) - 709871..711328 (-) 1458 WP_301401299.1 recombinase family protein -
  QYH60_RS03570 (QYH60_03570) - 711447..711986 (-) 540 WP_023164651.1 PH domain-containing protein -
  QYH60_RS03575 (QYH60_03575) - 712042..712626 (-) 585 WP_301401301.1 hypothetical protein -
  QYH60_RS03580 (QYH60_03580) - 712637..713059 (-) 423 WP_259752677.1 XRE family transcriptional regulator -
  QYH60_RS03585 (QYH60_03585) - 713252..713461 (+) 210 WP_023349177.1 helix-turn-helix transcriptional regulator -
  QYH60_RS03590 (QYH60_03590) - 713516..714229 (+) 714 WP_301401303.1 ORF6C domain-containing protein -
  QYH60_RS03595 (QYH60_03595) - 714245..714427 (+) 183 WP_003130605.1 hypothetical protein -
  QYH60_RS03600 (QYH60_03600) - 714424..714546 (+) 123 WP_023164646.1 hypothetical protein -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 40401.59 Da        Isoelectric Point: 10.3001

>NTDB_id=778160 QYH60_RS03555 WP_081042697.1 708647..709720(+) (comGB) [Lactococcus lactis subsp. lactis strain KMGR2-43]
MSLGHQKIGIKKLISYLQMDISHLLKPKGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKEFTLKMKEGLA
SGQVLSEILESLSFSKNVVTQLALVEVHGNLSGTMQLVEFHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQ
LNSGSNFATLLINHLPLVFFSFGAFIFLLTALSVTLFKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVE
LRQIINLMKKQKSRIFSEVGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEHFFTKID
RLMQLIQPLVFIFVALMIILLYAAMLLPIYSNMGSGI

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=778160 QYH60_RS03555 WP_081042697.1 708647..709720(+) (comGB) [Lactococcus lactis subsp. lactis strain KMGR2-43]
TTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTACTTACAAATGGATATCTCACACCTACTGAAGCC
AAAAGGGAAAAAATTAAGACTGATTAAACAAGCCAAACTTATCCAACTGATGGGAAATCTTTTGAATAGTGGATTTAATT
TAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAGAATTTACATTAAAAATGAAAGAAGGTCTCGCT
TCTGGTCAAGTTTTATCAGAGATTTTAGAAAGTCTTTCATTTTCTAAAAATGTGGTGACACAACTTGCTTTGGTAGAAGT
GCATGGTAACTTGTCAGGGACGATGCAATTGGTTGAATTTCATCTAAAAAAACAGCTTAAAGTTAAAAATAAATTAGTGG
AAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGGGCTTAAAAAATTATTTACTACCACAA
CTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTATTTTTTTCGTTTGGAGCTTTTATATT
TTTACTGACAGCTTTGTCAGTAACTCTTTTTAAACGAAAATCAGCAATAATGAATTTTACATTTCTTGTTAAATTACCTC
TGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGGGAAATCTAATTGCTCAAGGTGTTGAA
TTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAAGTTGGCAAGAATCTTGATTTAGAATT
GAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACCCGAGCTCTCATTAATGATTGAATATG
GAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTTGGGAACATTTTTTTACAAAGATAGAT
CGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTCGTAGCATTGATGATTATTTTGCTATATGCAGCCATGTTACT
CCCCATCTATTCAAATATGGGTTCTGGAATATAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(41-159)

Type II secretion system (T2SS), protein F (T2SSF)

(227-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

83.284

95.518

0.796
  comGB Streptococcus gordonii str. Challis substr. CH1

53.959

95.518

0.515
  comGB Streptococcus mutans UA140

53.959

95.518

0.515
  comGB Streptococcus mutans UA159

53.666

95.518

0.513
  comGB/cglB Streptococcus mitis SK321

51.343

93.838

0.482
  comGB/cglB Streptococcus pneumoniae TIGR4

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae Rx1

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae D39

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae R6

51.045

93.838

0.479
  comGB/cglB Streptococcus mitis NCTC 12261

50.448

93.838

0.473