Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   PML89_RS05610 Genome accession   NZ_CP116503
Coordinates   1188997..1189500 (+) Length   167 a.a.
NCBI ID   WP_126762176.1    Uniprot ID   A0AAE9XMW7
Organism   Vagococcus lutrae strain K136-2     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1161840..1199532 1188997..1189500 within 0


Gene organization within MGE regions


Location: 1161840..1199532
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PML89_RS05475 (PML89_05475) - 1161840..1162601 (-) 762 WP_023606897.1 ParA family protein -
  PML89_RS05480 (PML89_05480) rsmG 1162696..1163418 (-) 723 WP_023606898.1 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG -
  PML89_RS05485 (PML89_05485) - 1163582..1163830 (-) 249 WP_152514079.1 hypothetical protein -
  PML89_RS05490 (PML89_05490) - 1164499..1165437 (-) 939 WP_272157937.1 Abi family protein -
  PML89_RS05495 (PML89_05495) - 1166200..1166730 (-) 531 WP_252999045.1 hypothetical protein -
  PML89_RS05500 (PML89_05500) - 1166751..1167335 (-) 585 WP_272157939.1 hypothetical protein -
  PML89_RS05505 (PML89_05505) - 1167804..1169540 (-) 1737 WP_252999043.1 DNA primase family protein -
  PML89_RS05510 (PML89_05510) - 1169537..1170355 (-) 819 WP_252999042.1 bifunctional DNA primase/polymerase -
  PML89_RS05515 (PML89_05515) - 1170348..1170686 (-) 339 WP_252999041.1 hypothetical protein -
  PML89_RS05520 (PML89_05520) - 1170676..1170888 (-) 213 WP_252999040.1 hypothetical protein -
  PML89_RS05525 (PML89_05525) - 1170982..1171158 (-) 177 WP_252999039.1 hypothetical protein -
  PML89_RS05530 (PML89_05530) - 1171195..1171461 (-) 267 WP_252999038.1 DNA-binding protein -
  PML89_RS05535 (PML89_05535) - 1171618..1172235 (+) 618 WP_272157940.1 helix-turn-helix domain-containing protein -
  PML89_RS05540 (PML89_05540) - 1172280..1173449 (+) 1170 WP_272157941.1 site-specific integrase -
  PML89_RS05545 (PML89_05545) mnmG 1173520..1175427 (-) 1908 WP_272157942.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG -
  PML89_RS05550 (PML89_05550) mnmE 1175441..1176832 (-) 1392 WP_023606901.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  PML89_RS05555 (PML89_05555) jag 1177005..1177739 (-) 735 WP_126762187.1 RNA-binding cell elongation regulator Jag/EloR -
  PML89_RS05560 (PML89_05560) - 1177768..1178592 (-) 825 WP_023606903.1 YidC/Oxa1 family membrane protein insertase -
  PML89_RS05565 (PML89_05565) rnpA 1178611..1178958 (-) 348 WP_023606904.1 ribonuclease P protein component -
  PML89_RS05570 (PML89_05570) rpmH 1179098..1179232 (-) 135 WP_023606905.1 50S ribosomal protein L34 -
  PML89_RS05575 (PML89_05575) dnaA 1179821..1181164 (+) 1344 WP_023606906.1 chromosomal replication initiator protein DnaA -
  PML89_RS05580 (PML89_05580) dnaN 1181330..1182460 (+) 1131 WP_272158216.1 DNA polymerase III subunit beta -
  PML89_RS05585 (PML89_05585) yaaA 1182653..1182904 (+) 252 WP_023606923.1 S4 domain-containing protein YaaA -
  PML89_RS05590 (PML89_05590) recF 1182901..1184031 (+) 1131 WP_252999035.1 DNA replication/repair protein RecF -
  PML89_RS05595 (PML89_05595) gyrB 1184028..1185983 (+) 1956 WP_126762183.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  PML89_RS05600 (PML89_05600) gyrA 1186007..1188484 (+) 2478 WP_252999034.1 DNA gyrase subunit A -
  PML89_RS05605 (PML89_05605) rpsF 1188655..1188954 (+) 300 WP_126762178.1 30S ribosomal protein S6 -
  PML89_RS05610 (PML89_05610) ssbA 1188997..1189500 (+) 504 WP_126762176.1 single-stranded DNA-binding protein Machinery gene
  PML89_RS05615 (PML89_05615) rpsR 1189522..1189761 (+) 240 WP_126762174.1 30S ribosomal protein S18 -
  PML89_RS05620 (PML89_05620) rplI 1189906..1190358 (+) 453 WP_252999033.1 50S ribosomal protein L9 -
  PML89_RS05625 (PML89_05625) dnaB 1190388..1191749 (+) 1362 WP_023606931.1 replicative DNA helicase -
  PML89_RS05630 (PML89_05630) - 1191819..1193438 (-) 1620 WP_023606932.1 ABC-F family ATP-binding cassette domain-containing protein -
  PML89_RS05635 (PML89_05635) - 1193579..1194565 (-) 987 WP_376715796.1 polyprenyl synthetase family protein -
  PML89_RS05640 (PML89_05640) - 1194589..1195134 (-) 546 WP_252999031.1 Gx transporter family protein -
  PML89_RS05645 (PML89_05645) - 1195217..1197109 (-) 1893 WP_252999030.1 NAD(P)/FAD-dependent oxidoreductase -
  PML89_RS05650 (PML89_05650) pplA 1197369..1198298 (+) 930 WP_023606936.1 extracellular electron transfer flavoprotein PplA -
  PML89_RS05655 (PML89_05655) - 1198423..1199532 (+) 1110 WP_311878060.1 FAD:protein FMN transferase -

Sequence


Protein


Download         Length: 167 a.a.        Molecular weight: 18502.39 Da        Isoelectric Point: 4.9114

>NTDB_id=777678 PML89_RS05610 WP_126762176.1 1188997..1189500(+) (ssbA) [Vagococcus lutrae strain K136-2]
MINNVVLVGRLTKDPDLRYTANGVAVATFTLAIDRPFTNQSGNREADFINTVIWRKPAETLANYARKGTLIGVVGRIQTR
NYENQQGQRVYVTEVVADSFQFLESKSVNEQRRQGSGSDFGAQPNNNQSFNQSSSSMPQQGMPDFGRDSDPFASSAKIDI
SDDDLPF

Nucleotide


Download         Length: 504 bp        

>NTDB_id=777678 PML89_RS05610 WP_126762176.1 1188997..1189500(+) (ssbA) [Vagococcus lutrae strain K136-2]
ATGATTAATAATGTCGTACTAGTAGGTAGATTAACTAAAGATCCAGATTTGCGTTATACAGCAAACGGTGTCGCTGTTGC
AACATTCACCTTGGCAATCGATCGTCCATTTACAAATCAAAGTGGTAATCGTGAAGCAGATTTTATCAACACGGTGATTT
GGCGAAAGCCCGCAGAGACATTGGCTAATTATGCACGAAAAGGGACTCTGATTGGTGTGGTAGGACGTATTCAAACACGT
AACTATGAAAACCAACAAGGTCAACGTGTCTATGTGACAGAAGTGGTAGCAGATAGTTTCCAATTCTTAGAGTCGAAGTC
AGTCAATGAACAACGACGTCAAGGCTCTGGTTCAGATTTTGGTGCTCAACCTAATAATAACCAAAGTTTTAATCAGTCAT
CATCTTCAATGCCTCAACAAGGTATGCCGGATTTTGGTCGTGATTCAGACCCATTCGCATCATCAGCTAAGATAGATATC
TCAGATGATGATTTACCATTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

56.522

100

0.623

  ssb Latilactobacillus sakei subsp. sakei 23K

59.649

100

0.611

  ssbB Bacillus subtilis subsp. subtilis str. 168

61.321

63.473

0.389

  ssb Glaesserella parasuis strain SC1401

32.461

100

0.371