Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PL263_RS13055 Genome accession   NZ_CP116398
Coordinates   2909909..2910970 (-) Length   353 a.a.
NCBI ID   WP_140914059.1    Uniprot ID   -
Organism   Methylomonas sp. EFPC3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2904909..2915970
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PL263_RS13035 (PL263_13030) pilQ 2905912..2908164 (-) 2253 WP_278209779.1 type IV pilus secretin family protein -
  PL263_RS13040 (PL263_13035) - 2908169..2908720 (-) 552 WP_140914133.1 pilus assembly protein PilP -
  PL263_RS13045 (PL263_13040) - 2908720..2909340 (-) 621 WP_140914057.1 type 4a pilus biogenesis protein PilO -
  PL263_RS13050 (PL263_13045) - 2909337..2909909 (-) 573 WP_278209780.1 PilN domain-containing protein -
  PL263_RS13055 (PL263_13050) pilM 2909909..2910970 (-) 1062 WP_140914059.1 pilus assembly protein PilM Machinery gene
  PL263_RS13060 (PL263_13055) - 2911213..2913528 (+) 2316 WP_278209781.1 penicillin-binding protein 1A -
  PL263_RS13065 (PL263_13060) rpmE 2913589..2913789 (-) 201 WP_140914061.1 50S ribosomal protein L31 -
  PL263_RS13070 (PL263_13065) - 2913889..2915232 (-) 1344 WP_278209782.1 MFS transporter -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 37723.28 Da        Isoelectric Point: 4.7768

>NTDB_id=777134 PL263_RS13055 WP_140914059.1 2909909..2910970(-) (pilM) [Methylomonas sp. EFPC3]
MSWFSRNPSAVLGVDISTAAVKLLELSRAGARYKVESYAVVPLPQDAIVDKNITNVEQIGGAIKAAVKQSATKAKHASVA
VAGSSVMTKIISMPASLTDAEMEEQIMVEADQYIPYSLDEVNLDFEVQGVTKSNPDMVDVLLAASRRENIEDRVAALAYA
GLKTVVVDVEAFAMENAFSLLIDQLPEGIGGMTVAIADIGATMTSLNILHAGKTVYTREQGFGGKQLTEEIQRRYGLSYE
EAGLAKKHGGLPDNYAADVLEPFKKAMLQQIARSLQFFVSSSANRGVDALILAGGCASIAGLDKLVERDLGIPSYIANPF
INMALSNRVKPQSLSNDTPAMMIACGLALRSFD

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=777134 PL263_RS13055 WP_140914059.1 2909909..2910970(-) (pilM) [Methylomonas sp. EFPC3]
ATGAGCTGGTTCAGCAGGAATCCGTCGGCTGTATTGGGAGTGGATATCAGTACGGCGGCGGTCAAGTTGTTGGAGCTAAG
TCGGGCAGGTGCGCGATATAAAGTCGAGAGTTACGCCGTAGTGCCGCTACCCCAGGACGCCATCGTTGACAAGAATATCA
CTAATGTCGAGCAAATTGGCGGAGCGATCAAGGCAGCGGTCAAGCAATCGGCTACCAAGGCCAAGCACGCCAGCGTCGCT
GTTGCCGGCTCGTCGGTAATGACGAAGATCATATCGATGCCGGCTTCGCTGACCGATGCCGAGATGGAAGAACAGATCAT
GGTCGAAGCGGATCAGTACATTCCTTATTCGCTTGACGAGGTGAATTTGGATTTCGAGGTGCAAGGGGTAACCAAAAGCA
ACCCGGATATGGTGGATGTGCTGCTTGCGGCGTCCCGGCGGGAAAATATCGAAGACCGCGTTGCAGCACTGGCTTATGCC
GGCTTGAAAACGGTGGTGGTCGATGTCGAGGCATTTGCCATGGAAAATGCTTTTTCGCTGTTGATCGACCAACTGCCCGA
AGGGATTGGCGGTATGACTGTCGCGATCGCCGATATCGGCGCGACGATGACGTCGTTGAATATATTGCATGCCGGAAAGA
CGGTTTACACCCGTGAACAGGGTTTCGGAGGCAAACAATTGACCGAGGAAATTCAACGCCGTTACGGACTTTCCTACGAA
GAGGCGGGGTTGGCCAAGAAGCATGGCGGTTTGCCGGACAACTACGCGGCCGACGTACTGGAGCCTTTCAAAAAAGCCAT
GTTGCAGCAAATTGCTAGATCGCTCCAATTTTTCGTGTCTTCAAGCGCCAACCGTGGCGTCGACGCATTGATTCTGGCGG
GCGGTTGCGCGTCTATCGCGGGGTTGGACAAATTAGTCGAGCGCGATTTGGGCATACCTTCTTATATAGCCAATCCGTTT
ATCAATATGGCGCTGTCCAATCGGGTCAAGCCGCAAAGTCTCAGTAACGATACGCCGGCGATGATGATTGCTTGCGGCTT
GGCGTTAAGGAGTTTCGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.695

100

0.518
  comM Acinetobacter nosocomialis M2

51.412

100

0.516
  comM Acinetobacter baylyi ADP1

49.858

100

0.499
  pilM Legionella pneumophila strain ERS1305867

42.151

97.45

0.411