Detailed information NTG0776328

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PI990_RS12395
• Genome accession: NZ_CP116239
• Coordinates: 2669816..2671036 (+)
• Length: 406 a.a.
• NCBI ID: WP_031286771.1
• Uniprot ID: -
• Organism: Pseudomonas sp. TUM22785
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2664816..2676036

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PI990_RS12370 (PI990_12370)	-	2664948..2665829 (+)	882	WP_271660771.1	PhzF family phenazine biosynthesis protein	-
PI990_RS12375 (PI990_12375)	-	2665853..2666353 (+)	501	WP_184489396.1	DUF3291 domain-containing protein	-
PI990_RS12380 (PI990_12380)	-	2666376..2667593 (+)	1218	WP_271660772.1	winged helix-turn-helix domain-containing protein	-
PI990_RS12385 (PI990_12385)	-	2667656..2669245 (-)	1590	WP_031286787.1	ABC-F family ATPase	-
PI990_RS12390 (PI990_12390)	-	2669394..2669672 (-)	279	WP_021216621.1	peptidylprolyl isomerase	-
PI990_RS12395 (PI990_12395)	pilU	2669816..2671036 (+)	1221	WP_031286771.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PI990_RS12400 (PI990_12400)	-	2671281..2672039 (-)	759	WP_271660773.1	transporter substrate-binding domain-containing protein	-
PI990_RS12405 (PI990_12405)	-	2672115..2673068 (-)	954	WP_052351453.1	DUF4431 domain-containing protein	-
PI990_RS12410 (PI990_12410)	-	2673225..2675180 (-)	1956	WP_271660774.1	acetoacetate--CoA ligase	-

Sequence

Protein

• Length: 406 a.a.
• Molecular weight: 44787.12 Da
• Isoelectric Point: 6.4285

>NTDB_id=776328 PI990_RS12395 WP_031286771.1 2669816..2671036(+) (pilU) [Pseudomonas sp. TUM22785]
MDLPSMLKVLASQDGSDLYLSTGAPPCAKFNGVLKPLGTDPLKPGDVARIAESIMDPEQRGEFDRELEMNLAISLPNIGR
FRINIFKQRNEVSIVARNIKLDIPKFEDLKLPETLLKVVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTSDGKRRAAVEVLLGTSTISDLIKRGDFSSIKEIMEKSKALGMQTFDQAL
FDLVIEGSIQEEEAIKNADSANNLRLKLKLHRDGPGTRPTAAAPTPPPAPTPQATPTQDAASWGLELKLEDLPEEDPPPE
DPGKHY

Nucleotide

• Length: 1221 bp

>NTDB_id=776328 PI990_RS12395 WP_031286771.1 2669816..2671036(+) (pilU) [Pseudomonas sp. TUM22785]
ATGGATCTCCCCTCGATGCTCAAGGTTCTGGCCTCCCAGGATGGCTCGGACCTTTACCTTTCCACCGGCGCGCCGCCCTG
CGCCAAGTTCAACGGCGTGCTCAAGCCGCTGGGCACCGACCCGCTCAAGCCGGGCGACGTGGCGCGTATCGCCGAATCCA
TCATGGACCCGGAGCAGCGCGGTGAATTCGACCGTGAGCTGGAGATGAACCTGGCCATCTCCCTGCCCAACATCGGGCGG
TTCCGCATCAACATCTTCAAGCAGCGCAACGAGGTTTCCATCGTCGCGCGCAACATCAAGCTGGACATCCCCAAGTTCGA
AGACCTGAAGCTGCCCGAAACCCTGCTCAAGGTGGTGATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGTGGCACCGGCT
CCGGCAAGTCCACCTCCCTGGCCGCGCTGATCGACTACCGCAACCGCAACAGCGGCGGGCACATCATCACCATCGAAGAC
CCGGTGGAGTACGTGCACCGGCACAAGAAGTCCATCATCAACCAGCGTGAGGTGGGGGTGGACACCCGCAGCTTCCACGC
CGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAACACG
CCCTGGCCTTCGCCGACACCGGTCACCTGGCCATCTCCACCCTGCATGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAACGTCGCCCGCAACTGCTCAACGACCTGGGCAACAACCTCAAGGCCTTCGTTTCCCAGCG
TCTGGTGCGCACCTCGGACGGCAAGCGCCGCGCGGCGGTGGAAGTGCTACTGGGCACCTCCACCATCAGCGACCTGATCA
AGCGCGGCGATTTCAGCTCGATCAAGGAAATCATGGAGAAATCCAAGGCCCTGGGCATGCAGACCTTCGACCAGGCGCTG
TTCGATCTGGTGATCGAGGGTTCGATCCAGGAAGAGGAGGCGATCAAGAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GCTCAAGTTGCACCGCGACGGCCCCGGCACCCGGCCCACCGCCGCTGCACCGACCCCGCCTCCCGCACCGACGCCCCAGG
CCACGCCGACCCAGGATGCCGCCAGCTGGGGTCTGGAACTCAAGCTCGAAGACCTGCCCGAAGAAGATCCGCCGCCGGAA
GACCCCGGCAAGCACTACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (110-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	50.86	100	0.51
pilU	Acinetobacter baylyi ADP1	53.125	86.7	0.461
pilU	Vibrio cholerae strain A1552	51.275	86.946	0.446
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.86	95.074	0.369