Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   QVN08_RS03880 Genome accession   NZ_CP128888
Coordinates   797262..798755 (+) Length   497 a.a.
NCBI ID   WP_000249316.1    Uniprot ID   -
Organism   Escherichia coli strain TUM14697     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 792262..803755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QVN08_RS03860 (QVN08_03860) pilD 792910..793719 (+) 810 WP_000545349.1 prepilin peptidase PppA Machinery gene
  QVN08_RS03865 (QVN08_03865) gspS2 793785..794195 (+) 411 WP_001298257.1 type II secretion system pilot lipoprotein GspS-beta -
  QVN08_RS03870 (QVN08_03870) gspC 794213..795172 (+) 960 WP_001229917.1 type II secretion system protein GspC -
  QVN08_RS03875 (QVN08_03875) gspD 795202..797262 (+) 2061 WP_000498830.1 type II secretion system secretin GspD -
  QVN08_RS03880 (QVN08_03880) pilB/pilB1 797262..798755 (+) 1494 WP_000249316.1 type II secretion system ATPase GspE Machinery gene
  QVN08_RS03885 (QVN08_03885) gspF 798755..799978 (+) 1224 WP_001173414.1 type II secretion system inner membrane protein GspF -
  QVN08_RS03890 (QVN08_03890) gspG 799995..800450 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  QVN08_RS03895 (QVN08_03895) gspH 800454..801017 (+) 564 WP_001115113.1 type II secretion system minor pseudopilin GspH -
  QVN08_RS03900 (QVN08_03900) gspI 801014..801385 (+) 372 WP_000820087.1 type II secretion system minor pseudopilin GspI -
  QVN08_RS03905 (QVN08_03905) gspJ 801382..801987 (+) 606 WP_001312100.1 type II secretion system minor pseudopilin GspJ -
  QVN08_RS03910 (QVN08_03910) gspK 801984..802961 (+) 978 WP_000631648.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55194.36 Da        Isoelectric Point: 5.9081

>NTDB_id=775575 QVN08_RS03880 WP_000249316.1 797262..798755(+) (pilB/pilB1) [Escherichia coli strain TUM14697]
MVPVAQETSANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSLELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSVPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=775575 QVN08_RS03880 WP_000249316.1 797262..798755(+) (pilB/pilB1) [Escherichia coli strain TUM14697]
ATGGTGCCTGTAGCACAGGAAACCTCCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGCCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAAGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAACTGATGGAAGATCTCGGTTCTGCTGAGGATTTTTTTACCCTCGCAGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGCGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGCGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGTAAACTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTCCGTGTATCTACCATGCCTTCCGCCTGGGGGG
AACGGGTGGTGCTGCGACTGCTGGACAAAAACCAGGCTCGCCTGACGTTGGAGCGTCTGGGTTTAAGTCTTGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACCGGTTCCGGCAAAAGCACCAC
GCTGTACGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACATTCGCCCGTGGCCTGCGCGCAATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACGCTGCATACCAACACAGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGATTGCCGCCAGTCCGTGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCGTACCGTCACGCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGCACCGCTATCCATGAACTGATTGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.111

79.879

0.384
  pilB Acinetobacter baumannii D1279779

46.883

80.684

0.378
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

45.25

80.483

0.364