Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   PF996_RS09925 Genome accession   NZ_CP116014
Coordinates   1952616..1953050 (+) Length   144 a.a.
NCBI ID   WP_007611576.1    Uniprot ID   A0A172XIP3
Organism   Bacillus velezensis strain SRCM124633     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1947616..1958050
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF996_RS09920 (PF996_09920) - 1951116..1952327 (-) 1212 WP_025649439.1 cytochrome P450 family protein -
  PF996_RS09925 (PF996_09925) nucA/comI 1952616..1953050 (+) 435 WP_007611576.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  PF996_RS09930 (PF996_09930) - 1953110..1953865 (+) 756 WP_110085565.1 YoaK family protein -
  PF996_RS09935 (PF996_09935) - 1953899..1954261 (-) 363 WP_007410383.1 hypothetical protein -
  PF996_RS09940 (PF996_09940) - 1954453..1955781 (-) 1329 WP_106067939.1 S8 family peptidase -
  PF996_RS09945 (PF996_09945) - 1955960..1956193 (+) 234 WP_106067946.1 hypothetical protein -
  PF996_RS09950 (PF996_09950) - 1956449..1957156 (+) 708 WP_014417828.1 poly-gamma-glutamate hydrolase family protein -
  PF996_RS09955 (PF996_09955) - 1957217..1957669 (+) 453 WP_044801455.1 OsmC family protein -
  PF996_RS09960 (PF996_09960) - 1957707..1958036 (-) 330 WP_032865393.1 DMT family transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15528.47 Da        Isoelectric Point: 7.2418

>NTDB_id=775262 PF996_RS09925 WP_007611576.1 1952616..1953050(+) (nucA/comI) [Bacillus velezensis strain SRCM124633]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=775262 PF996_RS09925 WP_007611576.1 1952616..1953050(+) (nucA/comI) [Bacillus velezensis strain SRCM124633]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATACT
CCGACGGCACGAGAGTACTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172XIP3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486