Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   PF977_RS08315 Genome accession   NZ_CP116012
Coordinates   1599184..1599801 (+) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis strain SRCM124333     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1594184..1604801
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF977_RS08280 (PF977_08280) aroE 1594842..1595684 (+) 843 WP_015714291.1 shikimate dehydrogenase -
  PF977_RS08285 (PF977_08285) yhbY 1595678..1595968 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  PF977_RS08290 (PF977_08290) nadD 1595980..1596549 (+) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  PF977_RS08295 (PF977_08295) yqeK 1596539..1597099 (+) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  PF977_RS08300 (PF977_08300) rsfS 1597117..1597473 (+) 357 WP_014480315.1 ribosome silencing factor -
  PF977_RS08305 (PF977_08305) yqeM 1597470..1598213 (+) 744 WP_015714290.1 class I SAM-dependent DNA methyltransferase -
  PF977_RS08310 (PF977_08310) comER 1598279..1599100 (-) 822 WP_015714289.1 late competence protein ComER -
  PF977_RS08315 (PF977_08315) comEA 1599184..1599801 (+) 618 WP_004398514.1 competence protein ComEA Machinery gene
  PF977_RS08320 (PF977_08320) comEB 1599868..1600437 (+) 570 WP_015714288.1 ComE operon protein 2 -
  PF977_RS08325 (PF977_08325) comEC 1600434..1602764 (+) 2331 WP_074794436.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  PF977_RS08330 (PF977_08330) yqzM 1602804..1602938 (-) 135 WP_003229983.1 YqzM family protein -
  PF977_RS08335 (PF977_08335) - 1602979..1603128 (+) 150 WP_003229985.1 hypothetical protein -
  PF977_RS08340 (PF977_08340) holA 1603168..1604211 (+) 1044 WP_015714286.1 DNA polymerase III subunit delta -
  PF977_RS08345 (PF977_08345) rpsT 1604226..1604492 (-) 267 WP_003229989.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=775099 PF977_RS08315 WP_004398514.1 1599184..1599801(+) (comEA) [Bacillus subtilis strain SRCM124333]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=775099 PF977_RS08315 WP_004398514.1 1599184..1599801(+) (comEA) [Bacillus subtilis strain SRCM124333]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39