Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QU520_RS03950 Genome accession   NZ_CP128613
Coordinates   803919..804899 (+) Length   326 a.a.
NCBI ID   WP_010359873.1    Uniprot ID   -
Organism   Escherichia coli strain WL297     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 798919..809899
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QU520_RS03920 (QU520_03920) yggM 799524..800531 (+) 1008 WP_000745200.1 DUF1202 family protein -
  QU520_RS03925 (QU520_03925) hemW 800596..801732 (-) 1137 WP_000239967.1 radical SAM family heme chaperone HemW -
  QU520_RS03930 (QU520_03930) rdgB 801725..802318 (-) 594 WP_001174753.1 XTP/dITP diphosphatase -
  QU520_RS03935 (QU520_03935) yggU 802326..802616 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  QU520_RS03940 (QU520_03940) yggT 802613..803179 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  QU520_RS03945 (QU520_03945) yggS 803197..803901 (-) 705 WP_000997794.1 pyridoxal phosphate homeostasis protein -
  QU520_RS03950 (QU520_03950) pilT 803919..804899 (+) 981 WP_010359873.1 type IV pilus twitching motility protein PilT Machinery gene
  QU520_RS03955 (QU520_03955) ruvX 805073..805489 (-) 417 WP_289787417.1 Holliday junction resolvase RuvX -
  QU520_RS03960 (QU520_03960) yqgE 805489..806052 (-) 564 WP_001352952.1 YqgE/AlgH family protein -
  QU520_RS03965 (QU520_03965) gshB 806161..807111 (-) 951 WP_000593299.1 glutathione synthase -
  QU520_RS03970 (QU520_03970) rsmE 807124..807855 (-) 732 WP_001300912.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QU520_RS03975 (QU520_03975) endA 807935..808642 (-) 708 WP_000286512.1 deoxyribonuclease I -
  QU520_RS03980 (QU520_03980) yggI 808737..809234 (-) 498 WP_001296359.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36031.28 Da        Isoelectric Point: 5.9975

>NTDB_id=775083 QU520_RS03950 WP_010359873.1 803919..804899(+) (pilT) [Escherichia coli strain WL297]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGMSLALRLLPSHCPQLEQLGAPPLLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLVNTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VKEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=775083 QU520_RS03950 WP_010359873.1 803919..804899(+) (pilT) [Escherichia coli strain WL297]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGGATGGAAGCTGCGCCGTTTGATGCGCCGGACGTGGAAGAGCTATTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGTTAGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCGCAACGGCAAGGTATGTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCACCACCGTTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAAGATCCCGATGTGATATTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCACCTGGTGCTGGCAACTTTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCTGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCCGGGAGCTTGCGGGCAGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGGTTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCACATGTTATTCAAACTGGGCAACAAGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.379

97.239

0.393
  pilU Pseudomonas stutzeri DSM 10701

36.97

100

0.374
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362