Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   PF976_RS12540 Genome accession   NZ_CP116011
Coordinates   2416621..2417691 (-) Length   356 a.a.
NCBI ID   WP_013352872.1    Uniprot ID   A0A9P1JI17
Organism   Bacillus amyloliquefaciens strain SRCM124317     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2411621..2422691
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF976_RS12490 (PF976_12490) sipW 2411683..2412267 (-) 585 WP_013352863.1 signal peptidase I SipW -
  PF976_RS12495 (PF976_12495) tapA 2412239..2412910 (-) 672 WP_013352864.1 amyloid fiber anchoring/assembly protein TapA -
  PF976_RS12500 (PF976_12500) - 2413168..2413497 (+) 330 WP_013352865.1 DUF3889 domain-containing protein -
  PF976_RS12505 (PF976_12505) - 2413538..2413717 (-) 180 WP_013352866.1 YqzE family protein -
  PF976_RS12510 (PF976_12510) comGG 2413771..2414148 (-) 378 WP_013352867.1 competence type IV pilus minor pilin ComGG Machinery gene
  PF976_RS12515 (PF976_12515) comGF 2414150..2414650 (-) 501 WP_013352868.1 competence type IV pilus minor pilin ComGF -
  PF976_RS12520 (PF976_12520) comGE 2414559..2414873 (-) 315 WP_014470662.1 competence type IV pilus minor pilin ComGE Machinery gene
  PF976_RS12525 (PF976_12525) comGD 2414857..2415294 (-) 438 WP_013352869.1 competence type IV pilus minor pilin ComGD Machinery gene
  PF976_RS12530 (PF976_12530) comGC 2415284..2415550 (-) 267 WP_044051905.1 competence type IV pilus major pilin ComGC Machinery gene
  PF976_RS12535 (PF976_12535) comGB 2415597..2416634 (-) 1038 WP_013352871.1 competence type IV pilus assembly protein ComGB Machinery gene
  PF976_RS12540 (PF976_12540) comGA 2416621..2417691 (-) 1071 WP_013352872.1 competence type IV pilus ATPase ComGA Machinery gene
  PF976_RS12545 (PF976_12545) - 2417885..2418835 (-) 951 WP_013352873.1 magnesium transporter CorA family protein -
  PF976_RS12550 (PF976_12550) - 2418982..2420282 (+) 1301 Protein_2450 hemolysin family protein -
  PF976_RS12555 (PF976_12555) - 2420320..2421147 (-) 828 WP_013352875.1 STAS domain-containing protein -
  PF976_RS12565 (PF976_12565) - 2421360..2421740 (-) 381 WP_013352876.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  PF976_RS12570 (PF976_12570) - 2421976..2422221 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40430.67 Da        Isoelectric Point: 9.0345

>NTDB_id=775042 PF976_RS12540 WP_013352872.1 2416621..2417691(-) (comGA) [Bacillus amyloliquefaciens strain SRCM124317]
MENIETYSRNIINEAYMARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFPGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSTGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKTDNCSVYCSLYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=775042 PF976_RS12540 WP_013352872.1 2416621..2417691(-) (comGA) [Bacillus amyloliquefaciens strain SRCM124317]
ATGGAGAATATTGAAACATACAGCAGGAACATCATCAACGAGGCCTATATGGCAAGGGCTTCAGATATACACATTGTGCC
GAGGGAAAGGGATGCTTTCATTCATTTTCGCATCGGTCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAATTTTTATCAGCCATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGATCACTGTCT
CTGCCGCTTCCAACGGAAACCGTTCATTTGAGGATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
ATTGCCTAAAAGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCCGGGGGCAGGCGCCGCATTATTATCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTTTTTACCGGCCCGACCGGATCTGGAAAAACAACCACCCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTGGAAGATCCCGTAGAAACGAGAGATGAAGATGTTTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCACCGGTTTAAAAGCGATTTTAAGACATGACCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACAGGACACCTCGTACTGTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGGGTGAACATGACGGAAATTGAACAAACCGTTATTGC
CATCGCCGCTCAGCGTCTGGTTGATTTAACGTGTCCGTTTTGCAAAACGGACAACTGTTCCGTGTACTGCAGTCTATACA
GGCAGACACGCCGTGCTGGGATTTATGAACTGCTTTACGGAAAAAATCTCACCCAGTGCTTTCAAGAAGCAAAAGGGGAG
CACGCCAATTTTCAATATCAGACGCTTCGCAGGCTGATCAGAAAAGGCATCGCGCTCGGTTATGTCACGACTGATAATTA
TGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.506

97.753

0.376
  pilF Thermus thermophilus HB27

38

98.315

0.374