Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   PF975_RS02835 Genome accession   NZ_CP116010
Coordinates   555635..557020 (+) Length   461 a.a.
NCBI ID   WP_014419012.1    Uniprot ID   I2CAS2
Organism   Bacillus velezensis strain SRCM123815     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 550635..562020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF975_RS02815 (PF975_02815) - 551782..552429 (-) 648 WP_007614107.1 YigZ family protein -
  PF975_RS02820 (PF975_02820) degS 552652..553815 (+) 1164 WP_007407484.1 two-component sensor histidine kinase DegS Regulator
  PF975_RS02825 (PF975_02825) degU 553892..554581 (+) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  PF975_RS02830 (PF975_02830) - 554679..555527 (+) 849 WP_003151387.1 DegV family protein -
  PF975_RS02835 (PF975_02835) comFA 555635..557020 (+) 1386 WP_014419012.1 DEAD/DEAH box helicase Machinery gene
  PF975_RS02840 (PF975_02840) - 557078..557362 (+) 285 WP_014419011.1 late competence development ComFB family protein -
  PF975_RS02845 (PF975_02845) comFC 557368..558057 (+) 690 WP_197535984.1 ComF family protein Machinery gene
  PF975_RS02850 (PF975_02850) - 558131..558550 (+) 420 WP_014419009.1 TIGR03826 family flagellar region protein -
  PF975_RS02855 (PF975_02855) flgM 558620..558886 (+) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  PF975_RS02860 (PF975_02860) - 558901..559383 (+) 483 WP_088056401.1 flagellar protein FlgN -
  PF975_RS02865 (PF975_02865) flgK 559399..560916 (+) 1518 WP_014419008.1 flagellar hook-associated protein FlgK -
  PF975_RS02870 (PF975_02870) flgL 560928..561845 (+) 918 WP_014419007.1 flagellar hook-associated protein FlgL -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52599.04 Da        Isoelectric Point: 10.2804

>NTDB_id=774936 PF975_RS02835 WP_014419012.1 555635..557020(+) (comFA) [Bacillus velezensis strain SRCM123815]
MSEGASEFSEVRDFFYGRHLLRSEIPFSDQRIKRLTEKEYITAEPSIIRRKNRYVCQRCGQSDQASFAAFWAPSAKRQIT
YCRACVMMGRADELTSLYSWNQAPENSWEPVKLSWEGTLTDGQKRAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLNKAFEKVEVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKKAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
RQKLQLQLPVFLFVPSVHVLKKTTDYFRKLNIRAEGVHAEDTFRKDKVKRFRDGRLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVMFHFGITNGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=774936 PF975_RS02835 WP_014419012.1 555635..557020(+) (comFA) [Bacillus velezensis strain SRCM123815]
ATGTCCGAAGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCATTTATTAAGATCTGAGATACCGTT
TTCAGACCAGCGCATCAAGCGTTTAACAGAAAAAGAATATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCAGCTTTGCGGCATTTTGGGCGCCCTCTGCCAAAAGGCAGATTACC
TATTGCCGCGCCTGTGTCATGATGGGAAGGGCTGATGAACTGACATCATTATATTCTTGGAATCAAGCCCCTGAAAACAG
CTGGGAGCCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCGGGCGGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTGTTAGTATGGGCGGTTTGCGGATCGGGGAAAACAGAGATGCTGTTTCCGGGTATCGAGTTT
GCGCTGAATCACGGTTTGCGCGTTTGTGTCGCAACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAATAA
AGCATTTGAGAAAGTCGAAGTTTCTGCTTTGTACGGCGGCAGCGAGGACAAAGGCCGCTTGACTCCGCTGATGATTTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGACATTAAGATTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCGACTCCTTCAGA
CACATTAAAAAAGAAGGCTGAAGCCGGTTTGCTGAAAAGCGTCCGCATACCGGCCAGATATCATCGGAAACCCCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACGGATTGGGTC
CGCCAAAAACTTCAATTACAGCTGCCGGTTTTCTTATTCGTCCCGTCTGTACACGTATTGAAAAAGACAACGGATTATTT
TCGGAAATTAAACATACGGGCAGAAGGCGTACATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGATTCAGAGACG
GCCGGCTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTTACCGTGCCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCGCTCGTACAGATCGCGGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCGTCATGTTTCATTTCGGAATTACAAACGGAATGAAAAAAGCAAAAAAACATATAGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(118-266)

Helicase conserved C-terminal domain (Helicase_C)

(330-421)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2CAS2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

62.391

99.783

0.623