Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   QUS01_RS02560 Genome accession   NZ_CP128590
Coordinates   372600..373838 (+) Length   412 a.a.
NCBI ID   WP_289783775.1    Uniprot ID   -
Organism   Escherichia coli strain VG35A2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 367600..378838
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUS01_RS02535 (QUS01_02535) mrcA 367880..370432 (-) 2553 WP_000673804.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  QUS01_RS02540 (QUS01_02540) hofM 370552..371331 (+) 780 WP_001297394.1 DNA utilization protein HofM -
  QUS01_RS02545 (QUS01_02545) hofN 371331..371870 (+) 540 WP_001069315.1 DNA utilization protein HofN -
  QUS01_RS02550 (QUS01_02550) hofO 371854..372294 (+) 441 WP_001055760.1 DNA utilization protein HofO -
  QUS01_RS02555 (QUS01_02555) hofP 372284..372688 (+) 405 WP_001300917.1 DNA utilization protein HofP -
  QUS01_RS02560 (QUS01_02560) comE 372600..373838 (+) 1239 WP_289783775.1 DNA uptake porin HofQ Machinery gene
  QUS01_RS02565 (QUS01_02565) aroK 374239..374760 (+) 522 WP_000818618.1 shikimate kinase AroK -
  QUS01_RS02570 (QUS01_02570) aroB 374817..375905 (+) 1089 WP_000439846.1 3-dehydroquinate synthase -
  QUS01_RS02575 (QUS01_02575) damX 375997..377283 (+) 1287 WP_000343178.1 cell division protein DamX -
  QUS01_RS02580 (QUS01_02580) dam 377390..378226 (+) 837 WP_000742143.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44742.32 Da        Isoelectric Point: 6.3183

>NTDB_id=774667 QUS01_RS02560 WP_289783775.1 372600..373838(+) (comE) [Escherichia coli strain VG35A2]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSIAWQNNNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLALHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=774667 QUS01_RS02560 WP_289783775.1 372600..373838(+) (comE) [Escherichia coli strain VG35A2]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTCGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGGCAGGAAGGCAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATAACAATATCGCCCGCCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGTCTTTTGCTACGAGATAAC
AAAACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGGCCGATGCGCAACACGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGTAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGCTTGCTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTTACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  comE Haemophilus influenzae 86-028NP

36.927

100

0.391
  pilQ Vibrio campbellii strain DS40M4

38.005

100

0.388
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.53

100

0.376
  pilQ Vibrio cholerae strain A1552

37.53

100

0.376
  comE Glaesserella parasuis strain SC1401

35.952

100

0.367