Detailed information NTG0773842

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PGN41_RS02020
• Genome accession: NZ_CP115820
• Coordinates: 412529..413674 (+)
• Length: 381 a.a.
• NCBI ID: WP_111262597.1
• Uniprot ID: -
• Organism: Pseudomonas tohonis strain Q4-3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 407529..418674

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PGN41_RS01995	-	407802..408395 (-)	594	WP_173175076.1	YggT family protein	-
PGN41_RS02000	-	408546..409763 (-)	1218	WP_271104545.1	hypothetical protein	-
PGN41_RS02005	proC	409769..410587 (-)	819	WP_271104546.1	pyrroline-5-carboxylate reductase	-
PGN41_RS02010	-	410601..411296 (-)	696	WP_263152193.1	YggS family pyridoxal phosphate-dependent enzyme	-
PGN41_RS02015	pilT	411365..412399 (+)	1035	WP_111262598.1	type IV pilus twitching motility protein PilT	Machinery gene
PGN41_RS02020	pilU	412529..413674 (+)	1146	WP_111262597.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PGN41_RS02025	-	413607..415100 (-)	1494	WP_271104547.1	UdgX family uracil-DNA binding protein	-
PGN41_RS02030	-	415100..416314 (-)	1215	WP_271104548.1	putative DNA modification/repair radical SAM protein	-
PGN41_RS02035	-	416416..416811 (+)	396	WP_263152198.1	NINE protein	-
PGN41_RS02040	-	416924..418303 (+)	1380	WP_236208167.1	pyridoxal-phosphate dependent enzyme	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42490.78 Da
• Isoelectric Point: 6.5931

>NTDB_id=773842 PGN41_RS02020 WP_111262597.1 412529..413674(+) (pilU) [Pseudomonas tohonis strain Q4-3]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVTKNAMSPEQTRETVLAVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLDDLKLPEILKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEYIHQHQNCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYTLYSQGEITYEDALLYADSANDLRLMIKLGSETDGDHLTSMSGGLSLEVSEEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=773842 PGN41_RS02020 WP_111262597.1 412529..413674(+) (pilU) [Pseudomonas tohonis strain Q4-3]
ATGGAATTCGAGAAACTGTTGCGCCTGATGGTCGAGAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTCCCCCCGTC
GATGAAGGTCAACGGCAAGATCATGCCCGTGACCAAGAACGCCATGTCCCCGGAGCAGACCCGCGAGACCGTCCTCGCGG
TGATGAACGAGCAGCAGCGCCGCGACTTCGCCGAGAACCACGAGTGCAACTTCGCCATCAGCGCCCGTGGCATCGGTCGT
TTCCGCGTCAGCGCCTTTTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGACCAACATCCCCACCCTGGA
TGACCTCAAGCTGCCGGAGATCCTCAAGAAGCTGGCGCTGACCAAGCGTGGCCTGGTGCTGTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACAGCAGTGGCCACATCATCTCCATCGAAGAC
CCGATCGAATACATCCACCAGCACCAGAACTGCATCGTCACCCAGCGCGAGGTGGGCATCGACACCGAGTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAGGTGCGGACCCGCGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCCGCCGACCGGCAGCAGCAGGTGTGGATGGACCTATCCCTCAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCGGACGGCAAGGGCCGCCGCGCGGTGATCGAGGTGCTGATCAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTCCACGAGCTCAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACACCCTCTACTCCCAGGGCGAGATCACCTACGAAGACGCACTGCTCTACGCCGACTCGGCGAACGACCTGCGCCT
GATGATCAAGCTCGGTTCCGAGACCGACGGCGACCACCTCACGAGCATGAGCGGTGGCCTGTCGCTGGAAGTATCGGAGG
AAGACCCGGGCCGTCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (110-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	91.601	100	0.916
pilU	Acinetobacter baylyi ADP1	64.426	93.701	0.604
pilU	Vibrio cholerae strain A1552	58.857	91.864	0.541
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.155	88.189	0.381