Detailed information NTG0773417

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: O6P39_RS12305
• Genome accession: NZ_CP115669
• Coordinates: 2641623..2642846 (+)
• Length: 407 a.a.
• NCBI ID: WP_275611600.1
• Uniprot ID: -
• Organism: Pseudomonas sp. PSE14
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2636623..2647846

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
O6P39_RS12275 (O6P39_12275)	-	2636807..2637094 (+)	288	WP_275611595.1	hypothetical protein	-
O6P39_RS12280 (O6P39_12280)	-	2637153..2638445 (-)	1293	WP_275611596.1	OprD family porin	-
O6P39_RS12285 (O6P39_12285)	-	2638711..2639892 (-)	1182	WP_275611597.1	MFS transporter	-
O6P39_RS12290 (O6P39_12290)	-	2640234..2640788 (-)	555	WP_275611598.1	putative glycolipid-binding domain-containing protein	-
O6P39_RS12295 (O6P39_12295)	-	2640785..2641150 (-)	366	WP_275611599.1	MmcQ/YjbR family DNA-binding protein	-
O6P39_RS12300 (O6P39_12300)	-	2641158..2641436 (-)	279	WP_152223913.1	peptidylprolyl isomerase	-
O6P39_RS12305 (O6P39_12305)	pilU	2641623..2642846 (+)	1224	WP_275611600.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
O6P39_RS12310 (O6P39_12310)	-	2643419..2644669 (-)	1251	WP_275611601.1	OprD family porin	-
O6P39_RS12315 (O6P39_12315)	-	2645152..2646036 (+)	885	WP_275611602.1	DMT family transporter	-
O6P39_RS12320 (O6P39_12320)	-	2646157..2646975 (-)	819	WP_275611603.1	helix-turn-helix transcriptional regulator	-

Sequence

Protein

• Length: 407 a.a.
• Molecular weight: 44429.64 Da
• Isoelectric Point: 5.9218

>NTDB_id=773417 O6P39_RS12305 WP_275611600.1 2641623..2642846(+) (pilU) [Pseudomonas sp. PSE14]
MDIQSMLKILATQDGSDLYLSTGAPPCAKFNGVLKPLSNEPLKAGDVARIGYGLMDEEQRAEFERELEMNLAISVQGVGR
FRINLFKQRNEVSIVARNIKLDIPLFEDLKLPEVLLKVVMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PIEYVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTQDGKRRAAVEVLLGTATISDLIKRGDFSSIKEIMDKSRALGMQTFDQAL
FDLAVEGAISEEEAVKNADSANNLRLKLKLYKDNPAMVTNSAAATAASPAPAPAAAPAPAADSSSWGLELALEEIEAEDA
EGKPAGQ

Nucleotide

• Length: 1224 bp

>NTDB_id=773417 O6P39_RS12305 WP_275611600.1 2641623..2642846(+) (pilU) [Pseudomonas sp. PSE14]
ATGGATATCCAGTCGATGCTGAAGATCCTGGCCACCCAGGATGGTTCCGACCTCTACCTGTCCACCGGCGCGCCACCCTG
CGCCAAGTTCAACGGCGTGCTCAAGCCGCTGTCCAACGAGCCGCTCAAGGCGGGCGACGTGGCGCGCATCGGCTACGGCC
TGATGGACGAGGAACAGCGCGCCGAGTTCGAACGGGAACTGGAGATGAACCTGGCCATTTCGGTGCAGGGTGTCGGCCGC
TTCCGCATCAACCTGTTCAAGCAGCGCAACGAAGTGTCCATCGTGGCGCGCAACATCAAGCTGGACATCCCGCTGTTCGA
GGACCTGAAGCTGCCGGAAGTGCTGCTCAAGGTGGTGATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGCGGCACCGGCT
CGGGCAAGTCCACCTCCCTGGCGGCGCTGATCGACCACCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAAGAC
CCCATCGAGTACGTGCACCGGCACCGCAAGTCGATCATCAACCAGCGTGAGGTGGGCGTGGACACCCGCAGCTTCCATGC
CGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAACACG
CCCTGGCCTTCGCCGACACCGGGCACCTGGCGATCTCCACCCTGCACGCGAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGGCCGCAGTTGCTCAACGACCTGGGCAACAACCTCAAGGCCTTCGTTTCCCAGCG
CCTGGTGCGCACCCAGGACGGCAAGCGTCGCGCAGCGGTGGAAGTGCTGCTGGGCACCGCCACCATCAGCGACCTGATCA
AGCGCGGCGACTTCAGCTCGATCAAGGAAATCATGGACAAGTCCCGCGCCCTGGGCATGCAGACCTTCGACCAGGCGCTG
TTCGACCTGGCGGTGGAGGGGGCGATCAGCGAAGAAGAAGCGGTGAAGAACGCCGATTCGGCGAACAACCTGCGCCTGAA
GCTCAAGCTGTACAAGGACAACCCGGCCATGGTGACCAACTCCGCCGCAGCCACTGCCGCCAGCCCCGCGCCGGCACCCG
CCGCAGCGCCTGCACCGGCAGCGGACTCCTCCAGTTGGGGGCTGGAACTGGCGCTGGAAGAGATCGAGGCCGAAGACGCG
GAAGGCAAGCCGGCTGGGCAATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.98	86.241	0.491
pilU	Acinetobacter baylyi ADP1	53.693	86.486	0.464
pilU	Vibrio cholerae strain A1552	52.286	85.995	0.45
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.896	94.84	0.378