Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   QRX22_RS11065 Genome accession   NZ_CP128255
Coordinates   2296575..2297585 (+) Length   336 a.a.
NCBI ID   WP_000568522.1    Uniprot ID   Q8FGR3
Organism   Escherichia coli strain T309.Ta3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2291575..2302585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QRX22_RS11035 (QRX22_11035) nudB 2293298..2293750 (+) 453 WP_077248299.1 dihydroneopterin triphosphate diphosphatase -
  QRX22_RS11040 (QRX22_11040) yebC 2293779..2294519 (+) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  QRX22_RS11045 (QRX22_11045) ruvC 2294554..2295075 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  QRX22_RS11050 (QRX22_11050) yebB 2295077..2295679 (-) 603 WP_289201269.1 YebB family permuted papain-like enzyme -
  QRX22_RS11055 (QRX22_11055) yobI 2295751..2295816 (+) 66 WP_072127750.1 stress response small protein YobI -
  QRX22_RS11060 (QRX22_11060) ruvA 2295955..2296566 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  QRX22_RS11065 (QRX22_11065) ruvB 2296575..2297585 (+) 1011 WP_000568522.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  QRX22_RS11070 (QRX22_11070) znuB 2297732..2298517 (-) 786 WP_000571475.1 zinc ABC transporter permease subunit ZnuB -
  QRX22_RS11075 (QRX22_11075) znuC 2298514..2299269 (-) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  QRX22_RS11080 (QRX22_11080) znuA 2299348..2300280 (+) 933 WP_001347089.1 zinc ABC transporter substrate-binding protein ZnuA -
  QRX22_RS11085 (QRX22_11085) mepM 2300296..2301618 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37171.80 Da        Isoelectric Point: 4.7818

>NTDB_id=773345 QRX22_RS11065 WP_000568522.1 2296575..2297585(+) (ruvB) [Escherichia coli strain T309.Ta3]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TVRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=773345 QRX22_RS11065 WP_000568522.1 2296575..2297585(+) (ruvB) [Escherichia coli strain T309.Ta3]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCGCAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTATTGATTTTTGGTCCTCCGGGGTTGGGTAAAACGACGCTTGCCAATATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCAATGCTCACTAACCTTGAACCACA
CGATGTACTGTTTATTGATGAGATCCACCGTCTTTCACCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGATTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGTGCGCGCGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCTGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGCGGGCGTATGGCG
ACAGTGCGGGCGTGGAACCATTTCGGCATCACGCCACCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8FGR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

52.941

96.131

0.509
  ruvB Helicobacter pylori 26695

52.366

94.345

0.494