Detailed information NTG0773322

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PED36_RS03385
• Genome accession: NZ_CP115661
• Coordinates: 734402..735268 (-)
• Length: 288 a.a.
• NCBI ID: WP_010951890.1
• Uniprot ID: Q88Q64
• Organism: Pseudomonas putida strain PD689_delTT1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 729402..740268

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PED36_RS03350 (PED36_03350)	-	729874..731169 (-)	1296	WP_003247549.1	NAD(P)/FAD-dependent oxidoreductase	-
PED36_RS03355 (PED36_03355)	-	731254..731427 (-)	174	WP_008091951.1	DUF3094 domain-containing protein	-
PED36_RS03360 (PED36_03360)	-	731560..732189 (-)	630	WP_003247546.1	DUF1780 domain-containing protein	-
PED36_RS03365 (PED36_03365)	-	732245..732676 (-)	432	WP_049588119.1	hypothetical protein	-
PED36_RS03370 (PED36_03370)	-	732763..733470 (+)	708	WP_049588121.1	energy-coupling factor ABC transporter permease	-
PED36_RS03375 (PED36_03375)	yacG	733582..733782 (-)	201	WP_010951889.1	DNA gyrase inhibitor YacG	-
PED36_RS03380 (PED36_03380)	coaE	733779..734402 (-)	624	WP_004575721.1	dephospho-CoA kinase	-
PED36_RS03385 (PED36_03385)	pilD	734402..735268 (-)	867	WP_010951890.1	prepilin peptidase	Machinery gene
PED36_RS03390 (PED36_03390)	-	735271..736479 (-)	1209	WP_010951891.1	type II secretion system F family protein	-
PED36_RS03395 (PED36_03395)	-	736696..737106 (+)	411	WP_010951892.1	pilin	-
PED36_RS03405 (PED36_03405)	ltrA	737753..739174 (+)	1422	WP_138868000.1	group II intron reverse transcriptase/maturase	-
PED36_RS03410 (PED36_03410)	-	739448..740188 (-)	741	WP_010951894.1	cold shock domain-containing protein	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31830.86 Da
• Isoelectric Point: 7.5986

>NTDB_id=773322 PED36_RS03385 WP_010951890.1 734402..735268(-) (pilD) [Pseudomonas putida strain PD689_delTT1]
MTLWALLAEQPAYFLTLATVLGLLVGSFINVLVYRLPIMLERQWQREAQEVLGLPTTQHARFDLCLPASRCPHCAHRIRA
WENIPVISYLALGGRCSSCKNRISLRYPVVEVASALLSLVVAWRFGASVEALVALPLTWCLLALSLIDADHQLLPDVLVL
PTMWLGLIVNAFGIHVPLADALWGAVAGYLSLWTVYWVFRLVTGKEGMGYGDFKLMALIGAWGGWQVLPLTLLLSSVVGA
LFGLCLLRFRRDAMGTAIPFGPYLAIAGWIAVLWGDEIYASYMQLLGY

Nucleotide

• Length: 867 bp

>NTDB_id=773322 PED36_RS03385 WP_010951890.1 734402..735268(-) (pilD) [Pseudomonas putida strain PD689_delTT1]
ATGACTTTATGGGCTTTGCTGGCTGAACAGCCAGCGTATTTCCTCACCCTTGCCACGGTGCTCGGCCTGCTGGTGGGCAG
CTTTATCAACGTGCTGGTGTATCGCCTGCCGATCATGCTGGAGCGGCAATGGCAGCGTGAGGCGCAAGAGGTATTGGGGC
TGCCGACCACGCAGCACGCGCGTTTCGACCTGTGCCTGCCCGCCTCCCGGTGCCCACACTGCGCGCACCGGATTCGTGCG
TGGGAGAACATTCCGGTCATCAGCTACCTGGCCTTGGGCGGGCGCTGTTCGTCCTGCAAAAACCGCATCAGCCTGCGTTA
CCCGGTAGTGGAAGTGGCCAGCGCGCTGCTGTCGCTGGTAGTGGCCTGGCGCTTCGGCGCTTCGGTAGAGGCGTTGGTGG
CTCTGCCGCTGACCTGGTGCCTGCTGGCGCTGAGCCTGATCGATGCGGACCACCAGCTGCTGCCGGATGTGCTGGTGCTG
CCGACCATGTGGCTAGGGCTGATCGTCAATGCCTTTGGCATCCATGTGCCCCTGGCCGACGCACTGTGGGGCGCAGTGGC
CGGGTACCTCAGCCTGTGGACGGTGTACTGGGTGTTCAGGCTGGTCACCGGCAAGGAAGGCATGGGCTATGGCGATTTCA
AGCTGATGGCGCTGATCGGGGCGTGGGGGGGTTGGCAGGTGCTGCCGTTGACGCTGCTGTTGTCGTCGGTGGTGGGGGCG
CTGTTTGGCCTGTGCCTGCTGCGTTTTCGCCGGGATGCCATGGGCACGGCAATCCCGTTTGGCCCTTATCTGGCAATTGC
GGGGTGGATTGCCGTGCTCTGGGGTGATGAAATATACGCCTCATACATGCAACTGCTTGGATACTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-125)

• Type IV leader peptidase family (Peptidase_A24) (137-244)

3D structure

• AlphaFold DB: Q88Q64

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.347	96.181	0.503
pilD	Acinetobacter baumannii D1279779	49.822	97.569	0.486
pilD	Acinetobacter nosocomialis M2	49.11	97.569	0.479
pilD	Vibrio campbellii strain DS40M4	48.913	95.833	0.469
pilD	Neisseria gonorrhoeae MS11	48.327	93.403	0.451