Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PF550_RS04300 Genome accession   NZ_CP115641
Coordinates   888289..889407 (-) Length   372 a.a.
NCBI ID   WP_024432390.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain 2022CK-00251     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 852851..919985 888289..889407 within 0


Gene organization within MGE regions


Location: 852851..919985
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF550_RS04125 (PF550_04125) - 853252..853812 (-) 561 WP_002002990.1 hypothetical protein -
  PF550_RS04130 (PF550_04130) - 853903..854763 (-) 861 WP_000838103.1 pirin family protein -
  PF550_RS04135 (PF550_04135) hemJ 854934..855386 (-) 453 WP_000339890.1 protoporphyrinogen oxidase HemJ -
  PF550_RS04140 (PF550_04140) - 855405..856634 (-) 1230 WP_000832710.1 beta-ketoacyl-ACP synthase II -
  PF550_RS04145 (PF550_04145) - 856931..858256 (+) 1326 WP_000160004.1 EcsC family protein -
  PF550_RS04150 (PF550_04150) rpsL 858457..858831 (+) 375 WP_000246374.1 30S ribosomal protein S12 -
  PF550_RS04155 (PF550_04155) rpsG 858991..859461 (+) 471 WP_001138055.1 30S ribosomal protein S7 -
  PF550_RS04160 (PF550_04160) fusA 859640..861778 (+) 2139 WP_000113824.1 elongation factor G -
  PF550_RS04165 (PF550_04165) tuf 861873..863063 (+) 1191 WP_001029610.1 elongation factor Tu -
  PF550_RS04170 (PF550_04170) - 863267..864134 (+) 868 Protein_795 metal-dependent hydrolase -
  PF550_RS04175 (PF550_04175) - 864371..865246 (+) 876 WP_000992291.1 metal-dependent hydrolase -
  PF550_RS04180 (PF550_04180) rimI 865355..865810 (+) 456 WP_000621540.1 ribosomal protein S18-alanine N-acetyltransferase -
  PF550_RS04185 (PF550_04185) - 865855..866670 (-) 816 WP_024432392.1 arginyltransferase -
  PF550_RS04190 (PF550_04190) aat 866689..867420 (-) 732 WP_000854785.1 leucyl/phenylalanyl-tRNA--protein transferase -
  PF550_RS04195 (PF550_04195) trxB 867540..868487 (-) 948 WP_001276144.1 thioredoxin-disulfide reductase -
  PF550_RS04200 (PF550_04200) - 868757..871789 (+) 3033 WP_000127827.1 DNA translocase FtsK -
  PF550_RS04205 (PF550_04205) rhtC 871938..872558 (+) 621 WP_000959259.1 threonine export protein RhtC -
  PF550_RS04210 (PF550_04210) - 872601..872888 (-) 288 WP_001218560.1 PA4642 family protein -
  PF550_RS04215 (PF550_04215) minE 872972..873244 (-) 273 WP_000896934.1 cell division topological specificity factor MinE -
  PF550_RS04220 (PF550_04220) minD 873247..874059 (-) 813 WP_001074362.1 septum site-determining protein MinD -
  PF550_RS04225 (PF550_04225) minC 874130..874852 (-) 723 WP_000763675.1 septum site-determining protein MinC -
  PF550_RS04230 (PF550_04230) - 874974..876020 (-) 1047 WP_001181638.1 hypothetical protein -
  PF550_RS04235 (PF550_04235) - 876036..876962 (-) 927 WP_000100973.1 acyltransferase -
  PF550_RS04240 (PF550_04240) - 877207..877860 (+) 654 WP_001202415.1 OmpA family protein -
  PF550_RS04245 (PF550_04245) rep 878054..880093 (+) 2040 WP_281691119.1 DNA helicase Rep -
  PF550_RS04250 (PF550_04250) dut 880117..880569 (+) 453 WP_000868152.1 dUTP diphosphatase -
  PF550_RS04255 (PF550_04255) - 880769..882187 (+) 1419 WP_001102845.1 phosphomannomutase/phosphoglucomutase -
  PF550_RS04260 (PF550_04260) argB 882202..883110 (+) 909 WP_001135419.1 acetylglutamate kinase -
  PF550_RS04265 (PF550_04265) - 883231..884013 (+) 783 WP_000890284.1 GNAT family N-acetyltransferase -
  PF550_RS04270 (PF550_04270) - 884122..884970 (+) 849 WP_000336554.1 class II glutamine amidotransferase -
  PF550_RS04275 (PF550_04275) - 885219..885674 (+) 456 WP_000782976.1 bacteriohemerythrin -
  PF550_RS04280 (PF550_04280) - 885827..886894 (+) 1068 WP_029776981.1 membrane protein -
  PF550_RS04285 (PF550_04285) - 886894..887217 (+) 324 WP_000442585.1 RnfH family protein -
  PF550_RS04290 (PF550_04290) bamE 887272..887670 (-) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  PF550_RS04295 (PF550_04295) fur 887782..888219 (+) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  PF550_RS04300 (PF550_04300) pilU 888289..889407 (-) 1119 WP_024432390.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PF550_RS04305 (PF550_04305) pilT 889435..890472 (-) 1038 WP_000355489.1 type IV pilus twitching motility protein PilT Machinery gene
  PF550_RS04310 (PF550_04310) - 890599..891291 (+) 693 WP_024432389.1 YggS family pyridoxal phosphate-dependent enzyme -
  PF550_RS04315 (PF550_04315) - 891492..895088 (-) 3597 WP_000698797.1 AAA family ATPase -
  PF550_RS04320 (PF550_04320) - 895098..896366 (-) 1269 WP_032061991.1 exonuclease SbcCD subunit D -
  PF550_RS04325 (PF550_04325) - 896530..897003 (+) 474 WP_000974354.1 OsmC family protein -
  PF550_RS04330 (PF550_04330) - 897077..897454 (-) 378 WP_001091956.1 VOC family protein -
  PF550_RS04335 (PF550_04335) - 897834..898286 (+) 453 WP_000164152.1 ABZJ_00895 family protein -
  PF550_RS04340 (PF550_04340) - 898376..899281 (+) 906 WP_001039349.1 TIGR01777 family oxidoreductase -
  PF550_RS04345 (PF550_04345) - 899273..900388 (-) 1116 WP_000546678.1 FAD-dependent oxidoreductase -
  PF550_RS04350 (PF550_04350) hemB 900414..901427 (-) 1014 WP_004748084.1 porphobilinogen synthase -
  PF550_RS04355 (PF550_04355) - 901570..902067 (+) 498 WP_010590410.1 thioesterase family protein -
  PF550_RS04360 (PF550_04360) - 902337..903488 (+) 1152 WP_000128703.1 EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit -
  PF550_RS04365 (PF550_04365) - 903495..905018 (+) 1524 WP_000857095.1 DHA2 family efflux MFS transporter permease subunit -
  PF550_RS04370 (PF550_04370) ggt 905322..907307 (+) 1986 WP_001042935.1 gamma-glutamyltransferase -
  PF550_RS04380 (PF550_04380) - 908010..909239 (+) 1230 WP_032071232.1 tyrosine-type recombinase/integrase -
  PF550_RS04385 (PF550_04385) - 909389..910015 (+) 627 WP_000151566.1 hypothetical protein -
  PF550_RS04390 (PF550_04390) - 910143..910358 (+) 216 WP_000153154.1 hypothetical protein -
  PF550_RS04395 (PF550_04395) - 910361..910570 (+) 210 WP_001016479.1 helix-turn-helix domain-containing protein -
  PF550_RS04400 (PF550_04400) - 910567..911121 (+) 555 WP_002003158.1 BRO family protein -
  PF550_RS04405 (PF550_04405) - 911114..911479 (+) 366 WP_001046482.1 hypothetical protein -
  PF550_RS04410 (PF550_04410) - 911492..911794 (+) 303 WP_000787402.1 hypothetical protein -
  PF550_RS04415 (PF550_04415) - 911909..912187 (+) 279 WP_001064707.1 hypothetical protein -
  PF550_RS04420 (PF550_04420) - 912199..914664 (+) 2466 WP_281691120.1 DUF927 domain-containing protein -
  PF550_RS04425 (PF550_04425) - 914997..915200 (+) 204 WP_001984265.1 single-stranded DNA-binding protein -
  PF550_RS04430 (PF550_04430) - 915337..916779 (+) 1443 WP_000003532.1 hypothetical protein -
  PF550_RS04435 (PF550_04435) - 917122..917796 (+) 675 WP_000560650.1 DUF3800 domain-containing protein -
  PF550_RS04440 (PF550_04440) - 917796..917984 (+) 189 WP_000642900.1 hypothetical protein -
  PF550_RS04445 (PF550_04445) - 918040..918705 (+) 666 WP_000515964.1 hypothetical protein -
  PF550_RS19365 - 919177..919339 (+) 163 Protein_850 single-stranded DNA-binding protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41889.11 Da        Isoelectric Point: 6.6945

>NTDB_id=773135 PF550_RS04300 WP_024432390.1 888289..889407(-) (pilU) [Acinetobacter baumannii strain 2022CK-00251]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDFAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=773135 PF550_RS04300 WP_024432390.1 888289..889407(-) (pilU) [Acinetobacter baumannii strain 2022CK-00251]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
TCGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(31-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.661

100

0.769
  pilU Pseudomonas stutzeri DSM 10701

69.293

98.925

0.685
  pilU Vibrio cholerae strain A1552

52.973

99.462

0.527
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.197

93.011

0.392