Detailed information NTG0772939

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PF547_RS14785
• Genome accession: NZ_CP115629
• Coordinates: 3054117..3055235 (+)
• Length: 372 a.a.
• NCBI ID: WP_281725988.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain 2022CK-00337
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3049117..3060235

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PF547_RS14775 (PF547_14775)	-	3052233..3052925 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
PF547_RS14780 (PF547_14780)	pilT	3053052..3054089 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
PF547_RS14785 (PF547_14785)	pilU	3054117..3055235 (+)	1119	WP_281725988.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PF547_RS14790 (PF547_14790)	fur	3055305..3055742 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
PF547_RS14795 (PF547_14795)	bamE	3055854..3056252 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
PF547_RS14800 (PF547_14800)	-	3056307..3056630 (-)	324	WP_000442587.1	RnfH family protein	-
PF547_RS14805 (PF547_14805)	-	3056630..3057697 (-)	1068	WP_002019572.1	hypothetical protein	-
PF547_RS14810 (PF547_14810)	-	3057850..3058305 (-)	456	WP_000782976.1	bacteriohemerythrin	-
PF547_RS14815 (PF547_14815)	-	3058558..3059406 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41991.16 Da
• Isoelectric Point: 6.5679

>NTDB_id=772939 PF547_RS14785 WP_281725988.1 3054117..3055235(+) (pilU) [Acinetobacter baumannii strain 2022CK-00337]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRDIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=772939 PF547_RS14785 WP_281725988.1 3054117..3055235(+) (pilU) [Acinetobacter baumannii strain 2022CK-00337]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGACATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	52.973	99.462	0.527
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392