Detailed information NTG0772553

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PCP56_RS10530
• Genome accession: NZ_CP115481
• Coordinates: 2262140..2263288 (+)
• Length: 382 a.a.
• NCBI ID: WP_073625146.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain F002
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2257140..2268288

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP56_RS10505 (PCP56_10510)	-	2258291..2258587 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PCP56_RS10510 (PCP56_10515)	-	2258584..2259177 (-)	594	WP_003084544.1	YggT family protein	-
PCP56_RS10515 (PCP56_10520)	proC	2259188..2260009 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PCP56_RS10520 (PCP56_10525)	-	2260021..2260713 (-)	693	WP_015649764.1	YggS family pyridoxal phosphate-dependent enzyme	-
PCP56_RS10525 (PCP56_10530)	pilT	2260928..2261962 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PCP56_RS10530 (PCP56_10535)	pilU	2262140..2263288 (+)	1149	WP_073625146.1	type IV pilus ATPase PilU	Machinery gene
PCP56_RS10535 (PCP56_10540)	czcD	2263296..2264195 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PCP56_RS10540 (PCP56_10545)	-	2264349..2264753 (+)	405	WP_003084560.1	NINE protein	-
PCP56_RS10545 (PCP56_10550)	-	2264996..2266369 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
PCP56_RS10550 (PCP56_10555)	-	2266366..2267550 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42474.77 Da
• Isoelectric Point: 6.9321

>NTDB_id=772553 PCP56_RS10530 WP_073625146.1 2262140..2263288(+) (pilU) [Pseudomonas aeruginosa strain F002]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPGVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=772553 PCP56_RS10530 WP_073625146.1 2262140..2263288(+) (pilU) [Pseudomonas aeruginosa strain F002]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGGCGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	64.986	93.455	0.607
pilU	Vibrio cholerae strain A1552	60	91.623	0.55
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.773	88.743	0.38