Detailed information NTG0771664

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PCP07_RS28925
• Genome accession: NZ_CP115298
• Coordinates: 6252851..6253999 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain 37
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6247851..6258999

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP07_RS28900 (PCP07_28885)	-	6249002..6249298 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PCP07_RS28905 (PCP07_28890)	-	6249295..6249888 (-)	594	WP_003084544.1	YggT family protein	-
PCP07_RS28910 (PCP07_28895)	proC	6249899..6250720 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PCP07_RS28915 (PCP07_28900)	-	6250732..6251424 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PCP07_RS28920 (PCP07_28905)	pilT	6251639..6252673 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PCP07_RS28925 (PCP07_28910)	pilU	6252851..6253999 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PCP07_RS28930 (PCP07_28915)	czcD	6254007..6254906 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PCP07_RS28935 (PCP07_28920)	-	6255060..6255464 (+)	405	WP_003084560.1	NINE protein	-
PCP07_RS28940 (PCP07_28925)	-	6255707..6257080 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
PCP07_RS28945 (PCP07_28930)	-	6257077..6258261 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=771664 PCP07_RS28925 WP_003100959.1 6252851..6253999(+) (pilU) [Pseudomonas aeruginosa strain 37]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=771664 PCP07_RS28925 WP_003100959.1 6252851..6253999(+) (pilU) [Pseudomonas aeruginosa strain 37]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAAACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382