Detailed information NTG0771625

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PCP76_RS23705
• Genome accession: NZ_CP115297
• Coordinates: 5038540..5039688 (-)
• Length: 382 a.a.
• NCBI ID: WP_058018263.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain 135
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5033540..5044688

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP76_RS23685 (PCP76_23660)	-	5034278..5035462 (-)	1185	WP_033972291.1	cystathionine gamma-synthase	-
PCP76_RS23690 (PCP76_23665)	-	5035459..5036832 (-)	1374	WP_004365040.1	pyridoxal-phosphate dependent enzyme	-
PCP76_RS23695 (PCP76_23670)	-	5037075..5037479 (-)	405	WP_003084560.1	NINE protein	-
PCP76_RS23700 (PCP76_23675)	czcD	5037633..5038532 (+)	900	WP_024918014.1	CDF family zinc transporter CzcD	-
PCP76_RS23705 (PCP76_23680)	pilU	5038540..5039688 (-)	1149	WP_058018263.1	type IV pilus ATPase PilU	Machinery gene
PCP76_RS23710 (PCP76_23685)	pilT	5039866..5040900 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PCP76_RS23715 (PCP76_23690)	-	5041115..5041807 (+)	693	WP_073649040.1	YggS family pyridoxal phosphate-dependent enzyme	-
PCP76_RS23720 (PCP76_23695)	proC	5041819..5042640 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PCP76_RS23725 (PCP76_23700)	-	5042651..5043244 (+)	594	WP_003084544.1	YggT family protein	-
PCP76_RS23730 (PCP76_23705)	-	5043241..5043537 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42544.86 Da
• Isoelectric Point: 6.7792

>NTDB_id=771625 PCP76_RS23705 WP_058018263.1 5038540..5039688(-) (pilU) [Pseudomonas aeruginosa strain 135]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYIQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=771625 PCP76_RS23705 WP_058018263.1 5038540..5039688(-) (pilU) [Pseudomonas aeruginosa strain 135]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGTTGTCGCCGGAACAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGACTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTTTTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGATTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACATCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCCGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382