Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   PCP76_RS01565 Genome accession   NZ_CP115297
Coordinates   316452..317789 (-) Length   445 a.a.
NCBI ID   WP_073649712.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 135     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 315312..337105 316452..317789 within 0


Gene organization within MGE regions


Location: 315312..337105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP76_RS01560 (PCP76_01560) thiO 315312..316406 (+) 1095 WP_039028141.1 glycine oxidase ThiO -
  PCP76_RS01565 (PCP76_01565) pilR 316452..317789 (-) 1338 WP_073649712.1 two-component system response regulator PilR Regulator
  PCP76_RS01570 (PCP76_01570) pilS 317804..319396 (-) 1593 WP_039028118.1 two-component system sensor histidine kinase PilS Regulator
  PCP76_RS01575 (PCP76_01575) - 319386..319631 (-) 246 WP_003102591.1 PP0621 family protein -
  PCP76_RS01580 (PCP76_01580) - 319788..320813 (-) 1026 WP_033972916.1 outer membrane protein assembly factor BamD -
  PCP76_RS01585 (PCP76_01585) rluD 320959..321921 (+) 963 WP_033972915.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  PCP76_RS01590 (PCP76_01590) pgeF 321918..322646 (+) 729 WP_058179501.1 peptidoglycan editing factor PgeF -
  PCP76_RS01595 (PCP76_01595) clpB 322802..325366 (+) 2565 WP_003094682.1 ATP-dependent chaperone ClpB -
  PCP76_RS01615 (PCP76_01615) - 326036..326547 (-) 512 Protein_309 IS3 family transposase -
  PCP76_RS29775 - 327350..327592 (+) 243 Protein_310 hypothetical protein -
  PCP76_RS01625 (PCP76_01625) - 327951..328163 (+) 213 WP_211904283.1 fimbrial protein -
  PCP76_RS01630 - 328802..328999 (+) 198 WP_071546846.1 hypothetical protein -
  PCP76_RS01635 (PCP76_01630) - 329051..329812 (-) 762 Protein_313 IS3 family transposase -
  PCP76_RS01640 (PCP76_01635) - 329804..330103 (-) 300 Protein_314 ESPR domain-containing protein -
  PCP76_RS01645 (PCP76_01640) - 330119..331756 (-) 1638 WP_411567396.1 ShlB/FhaC/HecB family hemolysin secretion/activation protein -
  PCP76_RS01650 (PCP76_01645) zapE 332341..333399 (+) 1059 WP_033970453.1 cell division protein ZapE -
  PCP76_RS01655 (PCP76_01650) - 333438..334745 (-) 1308 WP_033970454.1 NAD(P)/FAD-dependent oxidoreductase -
  PCP76_RS01660 (PCP76_01655) - 334809..334979 (-) 171 WP_003094672.1 DUF3094 family protein -
  PCP76_RS01665 (PCP76_01660) - 335005..335454 (-) 450 WP_033970456.1 MOSC domain-containing protein -
  PCP76_RS01670 (PCP76_01665) - 335451..336080 (-) 630 WP_003094668.1 DUF1780 domain-containing protein -
  PCP76_RS01675 (PCP76_01670) - 336213..336638 (+) 426 WP_033976385.1 GNAT family N-acetyltransferase -
  PCP76_RS01680 (PCP76_01675) - 336635..337105 (+) 471 WP_073649710.1 FAD/FMN-containing dehydrogenase -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49736.12 Da        Isoelectric Point: 6.3896

>NTDB_id=771601 PCP76_RS01565 WP_073649712.1 316452..317789(-) (pilR) [Pseudomonas aeruginosa strain 135]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAREPFDLCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPKAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEVAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLAERI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADAPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=771601 PCP76_RS01565 WP_073649712.1 316452..317789(-) (pilR) [Pseudomonas aeruginosa strain 135]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAGCTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAGGCCCGCGAGTTGCTGGCCCGCGAGCCGTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAGACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTGGTGGCAACCGCCCTGCGCCTGCGCAACCCGAAGGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAATCACCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGCTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGCAGCGGCAAGGAGCTGGTGGCGCGCCTGATCCATGAGCAGGGGCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAACTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAGGCCGCCAGTGGCGGCACCCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGAGCGATCCAGGAAAAGGCCGTGCGCGCGGTCGGCGGCCAGCAGGAGGTCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCAGCCGAAGTCGGCGCCGGGCGCTTCCGCCAAGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCGCCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGAACGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGACTGACCGGCGACGCACAGGAGAAGCTGAAGAACTACCG
CTTTCCGGGCAACGTCCGCGAGCTGGAAAACATGCTGGAGCGCGCCTACACCCTGTGCGAAGACGACCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATGCGCCGGGTGCCAGCCAGGAAGGCGCGGCGAGCCTGAGCGAAATCGACAACCTCGAGGAC
TACCTGGAAGACATCGAGCGCAAGCTGATCATGCAGGCACTCGAGGAAACGCGCTGGAACCGCACCGCCGCGGCCCAGCG
CCTGGGCCTGACGTTCCGCTCGATGCGCTACCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(7-115)

Sigma-54 interaction domain (Sigma54_activat)

(135-301)

Bacterial regulatory protein, Fis family (HTH_8)

(402-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

99.775

100

0.998
  pilR Acinetobacter baumannii strain A118

50.763

100

0.524
  luxO Vibrio cholerae strain A1552

38.17

100

0.384