Detailed information NTG0077155

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: H7K70_RS18330
• Genome accession: NZ_AP022213
• Coordinates: 3952013..3953227 (-)
• Length: 404 a.a.
• NCBI ID: WP_182850468.1
• Uniprot ID: -
• Organism: Metapseudomonas otitidis strain WP8-S17-CRE-03
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3947013..3958227

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H7K70_RS18310 (WP8S17C03_35870)	-	3948884..3949675 (+)	792	WP_044409054.1	crotonase/enoyl-CoA hydratase family protein	-
H7K70_RS18315 (WP8S17C03_35880)	-	3949656..3950381 (+)	726	WP_182850465.1	alpha/beta fold hydrolase	-
H7K70_RS18320 (WP8S17C03_35890)	-	3950467..3951201 (+)	735	WP_232104203.1	ABC transporter substrate-binding protein	-
H7K70_RS18325 (WP8S17C03_35900)	-	3951333..3952001 (+)	669	WP_182850467.1	hypothetical protein	-
H7K70_RS18330 (WP8S17C03_35910)	pilU	3952013..3953227 (-)	1215	WP_182850468.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
H7K70_RS18335 (WP8S17C03_35920)	-	3953371..3953649 (+)	279	WP_044409048.1	peptidylprolyl isomerase	-
H7K70_RS29130 (WP8S17C03_35930)	-	3953768..3955072 (+)	1305	WP_044409045.1	PAS domain-containing methyl-accepting chemotaxis protein	-
H7K70_RS18345 (WP8S17C03_35940)	-	3955216..3956715 (+)	1500	WP_182850469.1	glycosyltransferase family 39 protein	-
H7K70_RS18350 (WP8S17C03_35950)	-	3956708..3957709 (+)	1002	WP_182850470.1	glycosyltransferase family 2 protein	-
H7K70_RS18355 (WP8S17C03_35960)	-	3957706..3958095 (+)	390	WP_182850471.1	GtrA family protein	-

Sequence

Protein

• Length: 404 a.a.
• Molecular weight: 44522.97 Da
• Isoelectric Point: 6.5154

>NTDB_id=77155 H7K70_RS18330 WP_182850468.1 3952013..3953227(-) (pilU) [Metapseudomonas otitidis strain WP8-S17-CRE-03]
MDLPSMLKVLASQDGSDLYLSTGAPPCAKFNGVLKPLSADPLKPGDVARIAESIMDPEQRVEFDRELEMNLAISVPNVGR
FRINIFKQRNEVSIVARNIKLDIPRFEDLKLPEVLLKVVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTSDGKRRAAVEVLLGTSTISDLIKRGDFSSIKEIMEKSKALGMQTFDQAL
FDLVVEGAISEDEAIKNADSANNLRLKLKLYRDGPAPAPTPAPLAAPTPPPAATRTQDAASWGLELKLEELPEETPPEDP
GKHF

Nucleotide

• Length: 1215 bp

>NTDB_id=77155 H7K70_RS18330 WP_182850468.1 3952013..3953227(-) (pilU) [Metapseudomonas otitidis strain WP8-S17-CRE-03]
ATGGATCTCCCTTCGATGCTGAAGGTCCTTGCCTCCCAGGATGGCTCGGACCTCTACCTGTCCACCGGCGCGCCGCCCTG
CGCCAAGTTCAACGGCGTGCTCAAGCCGCTGAGCGCCGACCCGCTCAAGCCGGGGGATGTCGCGCGTATCGCCGAGTCGA
TCATGGACCCGGAGCAGCGCGTCGAATTCGACCGTGAGCTGGAGATGAACCTGGCGATCTCGGTGCCCAACGTGGGGCGC
TTCCGCATCAACATCTTCAAACAGCGCAACGAGGTCTCCATCGTCGCGCGCAACATCAAGCTGGACATCCCCCGCTTCGA
GGACCTCAAGCTGCCCGAGGTGCTGCTCAAGGTGGTGATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGCGGCACCGGCT
CCGGCAAGTCCACCTCCCTGGCGGCACTGATCGACTACCGCAACCGCAACAGCGGCGGGCACATCATCACCATCGAGGAC
CCGGTGGAGTACGTGCACCGGCACAAGAAATCCATCATCAACCAGCGCGAGGTGGGCGTGGACACCCGCAGCTTCCACGC
CGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGTGAGATCCGCGACCGCGAGACCATGGAGCACG
CCCTGGCCTTCGCCGACACCGGTCACCTGGCCATTTCCACCCTGCACGCGAATAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAACGCCGGCCGCAGCTGCTCAACGACCTGGGCAACAACCTCAAGGCCTTCGTCTCCCAGCG
CCTGGTGCGCACTTCCGACGGCAAGCGTCGCGCGGCGGTGGAGGTGCTGTTGGGCACTTCCACCATCAGCGACCTGATCA
AGCGCGGTGACTTCAGCTCCATCAAGGAGATCATGGAGAAGTCCAAGGCCCTGGGCATGCAGACCTTCGACCAGGCGCTG
TTCGACCTGGTGGTGGAAGGCGCCATCAGCGAGGACGAGGCGATCAAGAACGCCGACTCCGCTAACAACCTGCGCCTTAA
GCTGAAGCTCTACCGCGACGGCCCGGCTCCCGCACCGACGCCCGCTCCCTTGGCTGCACCCACCCCGCCACCGGCCGCGA
CCCGTACCCAGGACGCAGCCAGTTGGGGGCTGGAGCTGAAGCTGGAGGAACTGCCCGAGGAAACGCCGCCGGAAGATCCG
GGCAAGCATTTCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.125	86.881	0.488
pilU	Acinetobacter baylyi ADP1	52.925	88.861	0.47
pilU	Vibrio cholerae strain A1552	52.125	87.376	0.455