Detailed information NTG0770748

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PCP74_RS01325
• Genome accession: NZ_CP115263
• Coordinates: 271781..272929 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain F023
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 266781..277929

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP74_RS01305 (PCP74_01305)	-	267519..268703 (-)	1185	WP_003117897.1	cystathionine gamma-synthase	-
PCP74_RS01310 (PCP74_01310)	-	268700..270073 (-)	1374	WP_015502220.1	pyridoxal-phosphate dependent enzyme	-
PCP74_RS01315 (PCP74_01315)	-	270316..270720 (-)	405	WP_003084560.1	NINE protein	-
PCP74_RS01320 (PCP74_01320)	czcD	270874..271773 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PCP74_RS01325 (PCP74_01325)	pilU	271781..272929 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PCP74_RS01330 (PCP74_01330)	pilT	273107..274141 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PCP74_RS01335 (PCP74_01335)	-	274356..275048 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PCP74_RS01340 (PCP74_01340)	proC	275060..275881 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PCP74_RS01345 (PCP74_01345)	-	275892..276485 (+)	594	WP_003084544.1	YggT family protein	-
PCP74_RS01350 (PCP74_01350)	-	276482..276778 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=770748 PCP74_RS01325 WP_003100959.1 271781..272929(-) (pilU) [Pseudomonas aeruginosa strain F023]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=770748 PCP74_RS01325 WP_003100959.1 271781..272929(-) (pilU) [Pseudomonas aeruginosa strain F023]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATTACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGATTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382