Detailed information NTG0770625

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PCP92_RS09455
• Genome accession: NZ_CP115259
• Coordinates: 2033722..2034870 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain F027
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2028722..2039870

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP92_RS09430 (PCP92_09435)	-	2029873..2030169 (-)	297	WP_031655578.1	DUF167 domain-containing protein	-
PCP92_RS09435 (PCP92_09440)	-	2030166..2030759 (-)	594	WP_003084544.1	YggT family protein	-
PCP92_RS09440 (PCP92_09445)	proC	2030770..2031591 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PCP92_RS09445 (PCP92_09450)	-	2031603..2032295 (-)	693	WP_003117895.1	YggS family pyridoxal phosphate-dependent enzyme	-
PCP92_RS09450 (PCP92_09455)	pilT	2032510..2033544 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PCP92_RS09455 (PCP92_09460)	pilU	2033722..2034870 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PCP92_RS09460 (PCP92_09465)	czcD	2034878..2035777 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PCP92_RS09465 (PCP92_09470)	-	2035932..2036336 (+)	405	WP_003084560.1	NINE protein	-
PCP92_RS09470 (PCP92_09475)	-	2036579..2037952 (+)	1374	WP_003117896.1	pyridoxal-phosphate dependent enzyme	-
PCP92_RS09475 (PCP92_09480)	-	2037949..2039133 (+)	1185	WP_003117897.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=770625 PCP92_RS09455 WP_003100959.1 2033722..2034870(+) (pilU) [Pseudomonas aeruginosa strain F027]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=770625 PCP92_RS09455 WP_003100959.1 2033722..2034870(+) (pilU) [Pseudomonas aeruginosa strain F027]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382