Detailed information NTG0770235

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PCP48_RS01815
• Genome accession: NZ_CP115248
• Coordinates: 391444..392592 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain F038
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 386444..397592

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP48_RS01790 (PCP48_01790)	-	387595..387891 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PCP48_RS01795 (PCP48_01795)	-	387888..388481 (-)	594	WP_003155373.1	YggT family protein	-
PCP48_RS01800 (PCP48_01800)	proC	388492..389313 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PCP48_RS01805 (PCP48_01805)	-	389325..390017 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PCP48_RS01810 (PCP48_01810)	pilT	390232..391266 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PCP48_RS01815 (PCP48_01815)	pilU	391444..392592 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PCP48_RS01820 (PCP48_01820)	czcD	392600..393499 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PCP48_RS01825 (PCP48_01825)	-	393653..394057 (+)	405	WP_003084560.1	NINE protein	-
PCP48_RS01830 (PCP48_01830)	-	394300..395673 (+)	1374	WP_003160915.1	pyridoxal-phosphate dependent enzyme	-
PCP48_RS01835 (PCP48_01835)	-	395670..396854 (+)	1185	WP_073652479.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=770235 PCP48_RS01815 WP_003100959.1 391444..392592(+) (pilU) [Pseudomonas aeruginosa strain F038]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=770235 PCP48_RS01815 WP_003100959.1 391444..392592(+) (pilU) [Pseudomonas aeruginosa strain F038]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382