Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   LPH31_RS09525 Genome accession   NZ_AP025220
Coordinates   1965340..1966350 (+) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain 2017.11.01CC     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1960340..1971350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPH31_RS09495 (VEGS20_A18370) nudB 1962064..1962516 (+) 453 WP_001300367.1 dihydroneopterin triphosphate diphosphatase -
  LPH31_RS09500 (VEGS20_A18380) yebC 1962545..1963285 (+) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  LPH31_RS09505 (VEGS20_A18390) ruvC 1963320..1963841 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  LPH31_RS09510 (VEGS20_A18400) yebB 1963843..1964445 (-) 603 WP_001024904.1 YebB family permuted papain-like enzyme -
  LPH31_RS09515 yobI 1964516..1964581 (+) 66 WP_072094247.1 stress response small protein YobI -
  LPH31_RS09520 (VEGS20_A18410) ruvA 1964720..1965331 (+) 612 WP_000580327.1 Holliday junction branch migration protein RuvA -
  LPH31_RS09525 (VEGS20_A18420) ruvB 1965340..1966350 (+) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  LPH31_RS09530 (VEGS20_A18430) znuB 1966497..1967282 (-) 786 WP_000571479.1 zinc ABC transporter permease subunit ZnuB -
  LPH31_RS09535 (VEGS20_A18440) znuC 1967279..1968034 (-) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  LPH31_RS09540 (VEGS20_A18450) znuA 1968113..1969045 (+) 933 WP_001300644.1 zinc ABC transporter substrate-binding protein ZnuA -
  LPH31_RS09545 (VEGS20_A18460) mepM 1969061..1970383 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=77006 LPH31_RS09525 WP_000568519.1 1965340..1966350(+) (ruvB) [Escherichia coli strain 2017.11.01CC]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=77006 LPH31_RS09525 WP_000568519.1 1965340..1966350(+) (ruvB) [Escherichia coli strain 2017.11.01CC]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATTTGGCTGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTTTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGACCTGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494

Multiple sequence alignment