Detailed information NTG0769384

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PCP85_RS01760
• Genome accession: NZ_CP115213
• Coordinates: 358445..359593 (-)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain F063
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 353445..364593

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP85_RS01740 (PCP85_01740)	-	354182..355366 (-)	1185	WP_023092310.1	cystathionine gamma-synthase	-
PCP85_RS01745 (PCP85_01745)	-	355363..356736 (-)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
PCP85_RS01750 (PCP85_01750)	-	356979..357383 (-)	405	WP_003084560.1	NINE protein	-
PCP85_RS01755 (PCP85_01755)	czcD	357538..358437 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PCP85_RS01760 (PCP85_01760)	pilU	358445..359593 (-)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
PCP85_RS01765 (PCP85_01765)	pilT	359771..360805 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PCP85_RS01770 (PCP85_01770)	-	361020..361712 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PCP85_RS01775 (PCP85_01775)	proC	361724..362545 (+)	822	WP_023092309.1	pyrroline-5-carboxylate reductase	-
PCP85_RS01780 (PCP85_01780)	-	362556..363149 (+)	594	WP_003084544.1	YggT family protein	-
PCP85_RS01785 (PCP85_01785)	-	363146..363442 (+)	297	WP_023980267.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=769384 PCP85_RS01760 WP_003110016.1 358445..359593(-) (pilU) [Pseudomonas aeruginosa strain F063]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=769384 PCP85_RS01760 WP_003110016.1 358445..359593(-) (pilU) [Pseudomonas aeruginosa strain F063]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382