Detailed information NTG0769281

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PCP41_RS03040
• Genome accession: NZ_CP115208
• Coordinates: 650473..651621 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain F066
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 645473..656621

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP41_RS03015 (PCP41_03015)	-	646624..646920 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PCP41_RS03020 (PCP41_03020)	-	646917..647510 (-)	594	WP_003084544.1	YggT family protein	-
PCP41_RS03025 (PCP41_03025)	proC	647521..648342 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PCP41_RS03030 (PCP41_03030)	-	648354..649046 (-)	693	WP_003117895.1	YggS family pyridoxal phosphate-dependent enzyme	-
PCP41_RS03035 (PCP41_03035)	pilT	649261..650295 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PCP41_RS03040 (PCP41_03040)	pilU	650473..651621 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
PCP41_RS03045 (PCP41_03045)	czcD	651629..652528 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PCP41_RS03050 (PCP41_03050)	-	652683..653087 (+)	405	WP_003084560.1	NINE protein	-
PCP41_RS03055 (PCP41_03055)	-	653330..654703 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
PCP41_RS03060 (PCP41_03060)	-	654700..655884 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=769281 PCP41_RS03040 WP_003110016.1 650473..651621(+) (pilU) [Pseudomonas aeruginosa strain F066]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=769281 PCP41_RS03040 WP_003110016.1 650473..651621(+) (pilU) [Pseudomonas aeruginosa strain F066]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCTGATCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382