Detailed information NTG0769215

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PCP91_RS03625
• Genome accession: NZ_CP115205
• Coordinates: 772727..773875 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain F068
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 767727..778875

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP91_RS03600 (PCP91_03605)	-	768878..769174 (-)	297	WP_023980267.1	DUF167 domain-containing protein	-
PCP91_RS03605 (PCP91_03610)	-	769171..769764 (-)	594	WP_003084544.1	YggT family protein	-
PCP91_RS03610 (PCP91_03615)	proC	769775..770596 (-)	822	WP_023092309.1	pyrroline-5-carboxylate reductase	-
PCP91_RS03615 (PCP91_03620)	-	770608..771300 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PCP91_RS03620 (PCP91_03625)	pilT	771515..772549 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PCP91_RS03625 (PCP91_03630)	pilU	772727..773875 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
PCP91_RS03630 (PCP91_03635)	czcD	773883..774782 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PCP91_RS03635 (PCP91_03640)	-	774937..775341 (+)	405	WP_003084560.1	NINE protein	-
PCP91_RS03640 (PCP91_03645)	-	775584..776957 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
PCP91_RS03645 (PCP91_03650)	-	776954..778138 (+)	1185	WP_023092310.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=769215 PCP91_RS03625 WP_003110016.1 772727..773875(+) (pilU) [Pseudomonas aeruginosa strain F068]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=769215 PCP91_RS03625 WP_003110016.1 772727..773875(+) (pilU) [Pseudomonas aeruginosa strain F068]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382