Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   LPH32_RS01425 Genome accession   NZ_AP025214
Coordinates   269537..270547 (+) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain 2017.09.02CC     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 264537..275547
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPH32_RS01395 (VEGS19_A02550) nudB 266261..266713 (+) 453 WP_020678468.1 dihydroneopterin triphosphate diphosphatase -
  LPH32_RS01400 (VEGS19_A02560) yebC 266742..267482 (+) 741 WP_000907248.1 YebC/PmpR family DNA-binding transcriptional regulator -
  LPH32_RS01405 (VEGS19_A02570) ruvC 267517..268038 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  LPH32_RS01410 (VEGS19_A02580) yebB 268040..268642 (-) 603 WP_001024932.1 YebB family permuted papain-like enzyme -
  LPH32_RS01415 yobI 268713..268778 (+) 66 WP_010723105.1 stress response small protein YobI -
  LPH32_RS01420 (VEGS19_A02590) ruvA 268917..269528 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  LPH32_RS01425 (VEGS19_A02600) ruvB 269537..270547 (+) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  LPH32_RS01430 (VEGS19_A02610) znuB 270694..271479 (-) 786 WP_000571479.1 zinc ABC transporter permease subunit ZnuB -
  LPH32_RS01435 (VEGS19_A02620) znuC 271476..272231 (-) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  LPH32_RS01440 (VEGS19_A02630) znuA 272310..273242 (+) 933 WP_001342995.1 zinc ABC transporter substrate-binding protein ZnuA -
  LPH32_RS01445 (VEGS19_A02640) mepM 273258..274580 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=76893 LPH32_RS01425 WP_000568519.1 269537..270547(+) (ruvB) [Escherichia coli strain 2017.09.02CC]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=76893 LPH32_RS01425 WP_000568519.1 269537..270547(+) (ruvB) [Escherichia coli strain 2017.09.02CC]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCCAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATTTGGCTGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACTCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGACCTGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494

Multiple sequence alignment