Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   PCQ86_RS19225 Genome accession   NZ_CP115182
Coordinates   3989505..3990086 (-) Length   193 a.a.
NCBI ID   WP_270174513.1    Uniprot ID   -
Organism   Diaphorobacter sp. ED-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3984505..3995086
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCQ86_RS19195 - 3984732..3985205 (+) 474 WP_103738377.1 hypothetical protein -
  PCQ86_RS19200 - 3985348..3985704 (+) 357 WP_041836445.1 phage holin family protein -
  PCQ86_RS19205 - 3985672..3987237 (-) 1566 WP_270174509.1 M48 family metalloprotease -
  PCQ86_RS19210 moaC 3987349..3987834 (+) 486 WP_270174510.1 cyclic pyranopterin monophosphate synthase MoaC -
  PCQ86_RS19215 - 3988006..3988797 (-) 792 WP_270174511.1 pilus assembly protein -
  PCQ86_RS19220 - 3988817..3989383 (-) 567 WP_270174512.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PCQ86_RS19225 pilE 3989505..3990086 (-) 582 WP_270174513.1 pilin Machinery gene
  PCQ86_RS19230 - 3990438..3991073 (-) 636 WP_270174514.1 FHA domain-containing protein -
  PCQ86_RS19235 - 3991178..3991993 (-) 816 WP_088888075.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  PCQ86_RS19240 - 3992276..3992959 (-) 684 WP_270174515.1 TerC family protein -
  PCQ86_RS19245 sucD 3993113..3994006 (-) 894 WP_015914606.1 succinate--CoA ligase subunit alpha -

Sequence

Protein


Download         Length: 193 a.a.        Molecular weight: 19532.53 Da        Isoelectric Point: 8.4851

>NTDB_id=768690 PCQ86_RS19225 WP_270174513.1 3989505..3990086(-) (pilE) [Diaphorobacter sp. ED-3]
MKRTLQQGFTLIELMIVVAIIGILAAVALPAYQDYSIRAKVSEGIIAASSAKAVLSEAFQTGGVTALDAAAAGINSTDFK
EKQSKYVANICVDQPPAVGANCAAHAGGATGPWQINMGIAATPANGIPTGMNGNVVSFLPLVQGAKPTATVSGAVDWACA
SSTQVTAKARFTGVTITVPGTPLPAKYAPAECR

Nucleotide


Download         Length: 582 bp        

>NTDB_id=768690 PCQ86_RS19225 WP_270174513.1 3989505..3990086(-) (pilE) [Diaphorobacter sp. ED-3]
ATGAAGCGTACCCTGCAACAAGGTTTCACCCTGATCGAATTGATGATCGTGGTGGCGATCATCGGTATTTTGGCTGCCGT
GGCTTTGCCTGCTTACCAAGACTACAGCATTCGGGCCAAAGTATCGGAAGGTATTATCGCTGCCTCTTCCGCTAAAGCGG
TACTGAGCGAGGCGTTTCAAACCGGTGGCGTGACTGCTCTGGACGCGGCTGCTGCGGGAATCAACTCCACCGACTTCAAG
GAAAAGCAGTCCAAATATGTGGCTAACATCTGTGTTGACCAACCGCCGGCCGTTGGTGCTAACTGTGCAGCTCACGCTGG
CGGTGCAACAGGACCTTGGCAAATCAACATGGGTATCGCTGCTACACCAGCTAACGGCATCCCCACTGGCATGAACGGTA
ACGTTGTCTCGTTCCTGCCATTGGTGCAAGGTGCAAAGCCGACAGCCACGGTGTCCGGTGCTGTTGATTGGGCTTGCGCT
AGCTCCACCCAAGTTACAGCTAAAGCACGCTTCACCGGGGTGACGATCACTGTACCAGGGACGCCTCTGCCCGCAAAGTA
CGCACCGGCTGAGTGCCGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(38-193)

Prokaryotic N-terminal methylation motif (N_methyl)

(6-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

51.471

100

0.544
  pilA Ralstonia pseudosolanacearum GMI1000

38.5

100

0.399
  pilA2 Legionella pneumophila str. Paris

38.298

97.409

0.373
  pilA2 Legionella pneumophila strain ERS1305867

37.766

97.409

0.368