NTDB

Overview

Name	ruvB	Type	Machinery gene
Locus tag	QN227_RS09205	Genome accession	NZ_CP126348
Coordinates	1922674..1923684 (+)	Length	336 a.a.
NCBI ID	WP_000568519.1	Uniprot ID	Q32HA1
Organism	Escherichia coli strain NN5
Function	homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1917674..1928684

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QN227_RS09175 (QN227_09175)	nudB	1919397..1919849 (+)	453	WP_077249130.1	dihydroneopterin triphosphate diphosphatase	-
QN227_RS09180 (QN227_09180)	yebC	1919878..1920618 (+)	741	WP_033557526.1	YebC/PmpR family DNA-binding transcriptional regulator	-
QN227_RS09185 (QN227_09185)	ruvC	1920653..1921174 (+)	522	WP_001295503.1	crossover junction endodeoxyribonuclease RuvC	-
QN227_RS09190 (QN227_09190)	yebB	1921176..1921778 (-)	603	WP_001024929.1	YebB family permuted papain-like enzyme	-
QN227_RS09195 (QN227_09195)	yobI	1921850..1921915 (+)	66	WP_072094247.1	stress response small protein YobI	-
QN227_RS09200 (QN227_09200)	ruvA	1922054..1922665 (+)	612	WP_000580328.1	Holliday junction branch migration protein RuvA	-
QN227_RS09205 (QN227_09205)	ruvB	1922674..1923684 (+)	1011	WP_000568519.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
QN227_RS09210 (QN227_09210)	znuB	1923831..1924616 (-)	786	WP_000571465.1	zinc ABC transporter permease subunit ZnuB	-
QN227_RS09215 (QN227_09215)	znuC	1924613..1925368 (-)	756	WP_000202996.1	zinc ABC transporter ATP-binding protein ZnuC	-
QN227_RS09220 (QN227_09220)	znuA	1925447..1926379 (+)	933	WP_001342995.1	zinc ABC transporter substrate-binding protein ZnuA	-
QN227_RS09225 (QN227_09225)	mepM	1926395..1927717 (+)	1323	WP_001184045.1	murein DD-endopeptidase MepM	-

Sequence

Protein

Download Length: 336 a.a. Molecular weight: 37173.77 Da Isoelectric Point: 4.7818

                                >NTDB_id=767274 QN227_RS09205 WP_000568519.1 1922674..1923684(+) (ruvB) [Escherichia coli strain NN5]

MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG

VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA

TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG

TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA

TTRAWNHFGITPPEMP

Nucleotide

Download Length: 1011 bp

                                >NTDB_id=767274 QN227_RS09205 WP_000568519.1 1922674..1923684(+) (ruvB) [Escherichia coli strain NN5]

ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT

ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG

CCCTCGATCATCTGTTGATTTTCGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC

GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA

TGACGTGTTGTTTATTGATGAGATCCACCGTCTTTCGCCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGACTACC

AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA

ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAGCGTCTGGAGTTTTATCAGGTGCC

GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC

GTCGAGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC

ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA

GTTATTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC

GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG

ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	60.486	97.917	0.592
ruvB	Streptococcus pneumoniae TIGR4	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae R6	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae D39	58.934	94.94	0.56
ruvB	Synechocystis sp. PCC 6803	53.251	96.131	0.512
ruvB	Helicobacter pylori 26695	52.038	94.94	0.494

Detailed information NTG0767274