Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   O5O48_RS03135 Genome accession   NZ_CP114836
Coordinates   695753..696817 (-) Length   354 a.a.
NCBI ID   WP_228245562.1    Uniprot ID   -
Organism   Halopseudomonas sp. MFKK-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 690753..701817
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O5O48_RS03110 aroK 691321..691830 (-) 510 WP_228245559.1 shikimate kinase AroK -
  O5O48_RS03115 pilQ 691836..693971 (-) 2136 WP_269688125.1 type IV pilus secretin PilQ Machinery gene
  O5O48_RS03120 pilP 694028..694540 (-) 513 WP_228245571.1 type 4a pilus biogenesis lipoprotein PilP -
  O5O48_RS03125 pilO 694555..695178 (-) 624 WP_088277117.1 type 4a pilus biogenesis protein PilO -
  O5O48_RS03130 - 695175..695753 (-) 579 WP_233048949.1 PilN domain-containing protein -
  O5O48_RS03135 pilM 695753..696817 (-) 1065 WP_228245562.1 pilus assembly protein PilM Machinery gene
  O5O48_RS03140 - 697002..699401 (+) 2400 WP_269688126.1 penicillin-binding protein 1A -
  O5O48_RS03145 - 699356..700306 (-) 951 WP_228247636.1 LysR family transcriptional regulator -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37972.49 Da        Isoelectric Point: 4.3061

>NTDB_id=767219 O5O48_RS03135 WP_228245562.1 695753..696817(-) (pilM) [Halopseudomonas sp. MFKK-1]
MLGLFKKKANTLLGIDISSTTVKLLELSRSGGRYKVEAYAVEPLPPASVVEKNIVELEGVGQALEKVLARSKSSLRQAAV
AVAGSAVITKVIEMDAGLDPDEMEEQIKIEADQYIPYPLDEVAIDFEVQGVSPRNPAREEVLLAACRRENVDIREAALAL
AGLTAKIVEVEAYAMERACELVIGQLDAGNDDLTIAVIDIGATMMTLSVLSGGRTIYTREQLFGGKQLTDEIQRRYGLSQ
DEAGLAKKQGGLPDDYEVEVLGPFKDAVVQQVSRSLQFFFAGGQYNDVDYILLAGGTASIPGLDQLVQQKIGTTTLVANP
FANMTLSNKVDAVALSNDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=767219 O5O48_RS03135 WP_228245562.1 695753..696817(-) (pilM) [Halopseudomonas sp. MFKK-1]
GTGCTAGGGCTCTTCAAGAAAAAAGCGAATACGCTGCTGGGCATCGACATCAGCTCCACCACCGTGAAGCTGCTCGAACT
CAGCCGCTCCGGTGGCCGCTACAAGGTCGAGGCCTACGCGGTCGAACCTCTCCCTCCCGCTTCGGTAGTGGAGAAGAACA
TTGTCGAACTCGAAGGCGTCGGTCAGGCGCTGGAGAAGGTACTGGCGCGATCGAAATCGTCGCTCAGGCAGGCCGCAGTG
GCGGTAGCCGGATCCGCGGTGATCACCAAGGTGATCGAGATGGATGCCGGGCTTGATCCTGACGAGATGGAAGAACAGAT
CAAGATCGAAGCCGACCAGTACATCCCCTACCCGCTGGACGAGGTTGCCATAGACTTCGAAGTCCAGGGCGTTTCCCCCC
GCAATCCCGCCAGAGAAGAAGTGTTGCTCGCTGCCTGTCGCCGTGAAAATGTCGATATCCGCGAAGCCGCACTGGCACTT
GCCGGTCTGACCGCCAAGATTGTCGAGGTCGAGGCTTACGCGATGGAGCGTGCCTGCGAGCTTGTTATCGGGCAGCTGGA
TGCGGGCAACGACGACCTGACCATCGCGGTGATCGACATCGGTGCAACCATGATGACCCTGAGTGTGCTCAGTGGTGGTC
GTACCATTTATACCCGCGAGCAGCTGTTTGGCGGCAAGCAACTGACGGATGAGATTCAGCGCCGTTACGGCTTGTCCCAG
GACGAGGCCGGGCTGGCCAAGAAGCAGGGCGGTCTTCCCGACGATTACGAAGTCGAGGTGCTGGGTCCCTTCAAGGATGC
AGTGGTGCAGCAGGTATCTCGATCGCTGCAGTTCTTCTTTGCCGGCGGGCAATACAACGACGTCGACTACATCTTGCTGG
CCGGTGGTACCGCTTCGATTCCAGGGCTGGATCAGTTGGTACAGCAGAAAATTGGTACCACCACTCTGGTTGCCAACCCC
TTTGCAAACATGACCCTGTCAAACAAGGTTGATGCGGTTGCCCTGAGCAATGACGCGCCCGCCCTGATGATTGCGTGTGG
TCTGGCAATGAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

53.107

100

0.531
  pilM Legionella pneumophila strain ERS1305867

46.197

100

0.463