Detailed information NTG0766834

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: O4M77_RS07640
• Genome accession: NZ_CP114606
• Coordinates: 1636287..1637465 (-)
• Length: 392 a.a.
• NCBI ID: WP_323713278.1
• Uniprot ID: A0AAU0NBQ8
• Organism: Acinetobacter sp. YWS30-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1631287..1642465

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
O4M77_RS07620 (O4M77_07605)	-	1631700..1632728 (+)	1029	WP_313482804.1	AraC family transcriptional regulator	-
O4M77_RS07625 (O4M77_07610)	gcvH	1632811..1633185 (+)	375	WP_104426083.1	glycine cleavage system protein GcvH	-
O4M77_RS07630 (O4M77_07615)	-	1633438..1634613 (+)	1176	WP_323713277.1	MFS transporter	-
O4M77_RS07635 (O4M77_07620)	pilU	1634687..1635883 (-)	1197	WP_005235842.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
O4M77_RS07640 (O4M77_07625)	pilU	1636287..1637465 (-)	1179	WP_323713278.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
O4M77_RS07645 (O4M77_07630)	-	1637704..1638753 (-)	1050	WP_180011009.1	3-deoxy-7-phosphoheptulonate synthase	-
O4M77_RS07650 (O4M77_07635)	-	1639306..1639836 (-)	531	WP_312087321.1	hypothetical protein	-
O4M77_RS07655 (O4M77_07640)	-	1640045..1641157 (-)	1113	WP_323713279.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-

Sequence

Protein

• Length: 392 a.a.
• Molecular weight: 44254.70 Da
• Isoelectric Point: 6.3715

>NTDB_id=766834 O4M77_RS07640 WP_323713278.1 1636287..1637465(-) (pilU) [Acinetobacter sp. YWS30-1]
MYTTELLDEARKFMHHMLTKVVEYGGSDLFITADFPPSIKQQGLMKPFGQQKLTADKTRLFAYALMNERQIQEFESVMEC
NFSISIPGVSRFRVNVFQQQHNVGMVIRIITTDIPSFEQLGLPASLQQVIMQKRGLVLVVGATGVGKSTTLAAMIDYRNT
HTEGHIITIEDPVEYVYQHKKSMITQREIGVDCVSWHNALINTMRQAPDVIVIGEIRDTDSMEHAIAFAESGHLCLATLH
SNNADQALDRIINFFPEERRNQLLLDLSANIKAVISQRLIRTEDGKGRRAAVEILQNTPLVSDLIRKGEFHELKTIMARS
RELGMQTFDQALFDLYNQGIISYEEALHNADSSNELRLQIKLKSSRINPVLQAENAQISLLEQSGSRVLSPD

Nucleotide

• Length: 1179 bp

>NTDB_id=766834 O4M77_RS07640 WP_323713278.1 1636287..1637465(-) (pilU) [Acinetobacter sp. YWS30-1]
ATGTATACAACTGAATTACTCGATGAAGCACGTAAATTTATGCACCATATGCTGACCAAAGTCGTGGAATATGGTGGTTC
CGATCTTTTTATCACAGCTGATTTCCCGCCTAGTATCAAACAGCAGGGGTTAATGAAACCGTTCGGCCAGCAGAAACTTA
CAGCCGATAAGACCCGTTTGTTTGCTTATGCCTTGATGAATGAGCGACAGATTCAGGAATTTGAATCGGTGATGGAATGC
AATTTTAGTATTAGCATCCCGGGCGTGTCGCGCTTCCGGGTTAATGTCTTTCAGCAACAGCACAATGTAGGCATGGTGAT
TCGAATCATTACGACAGATATTCCCAGCTTTGAGCAACTAGGCTTACCAGCTTCATTGCAACAGGTCATTATGCAGAAAC
GTGGTCTGGTGCTGGTAGTTGGTGCAACGGGTGTCGGAAAATCAACTACGTTGGCAGCCATGATTGATTACCGTAATACG
CATACTGAGGGGCATATTATTACGATCGAAGATCCGGTGGAATATGTCTATCAGCATAAAAAGTCGATGATTACCCAGCG
GGAAATTGGTGTGGATTGTGTTTCCTGGCACAATGCACTGATTAATACCATGCGGCAGGCACCTGACGTCATCGTGATTG
GTGAAATCCGCGATACAGACAGTATGGAGCATGCAATTGCCTTTGCTGAATCTGGCCATTTATGTCTTGCAACACTCCAT
TCTAATAATGCCGATCAGGCACTGGACCGGATTATTAACTTCTTTCCAGAAGAACGCCGTAATCAGTTACTGCTAGATTT
ATCAGCTAATATCAAAGCCGTGATTTCTCAGCGGCTAATTCGCACTGAAGATGGTAAAGGTCGTCGGGCTGCGGTAGAAA
TTCTGCAAAATACCCCTTTAGTTTCTGACCTGATTCGTAAAGGTGAATTCCATGAACTTAAAACGATTATGGCTCGTTCG
CGGGAACTGGGCATGCAAACCTTTGATCAGGCATTATTTGATTTATATAACCAAGGCATTATTTCTTATGAAGAAGCCTT
GCATAATGCAGACTCCAGCAATGAATTACGTTTGCAAATCAAGCTGAAGAGCAGTCGTATTAATCCTGTTTTACAGGCAG
AAAATGCACAGATTTCACTTCTGGAACAGTCCGGATCGCGTGTACTTTCTCCTGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (64-282)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	59.77	88.776	0.531
pilU	Acinetobacter baylyi ADP1	54.648	90.561	0.495
pilU	Vibrio cholerae strain A1552	52.113	90.561	0.472
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.353	86.735	0.367